PMID: 9530999Apr 8, 1998Paper

Do aluminium and/or glutamate induce Alz-50 reactivity? A light microscopic immunohistochemical study

Journal of Neurocytology
K R Jones, D E Oorschot

Abstract

Alzheimer's disease is thought to be characterized by conformational and phosphorylation changes in tau protein, leading to the formation of aggregations of paired helical filaments within neurons. Potential agents for inducing conformational changes in tau, namely aluminium and glutamate, were investigated in this study. Explant cultures of cortical neurons were established from embryonic day 17 rat fetuses. Cultures were exposed to aluminium, glutamate, aluminium/glutamate, aluminium/citric acid and citric acid (since aluminium is thought to enter cells via the transferrin receptor by complexing with citric acid) from 7-12 days in vitro. Control explants were exposed to basal medium only. On day 12, explants were paraffin-embedded. Four-six explants were serially sectioned per condition. For each explant, every 10th and adjacent 4 microm section were randomly selected and processed, with controls, for: (1) alcoholic morin histochemistry (to detect intracellular aluminium), (2) Perls' iron histochemistry (to control for the morin stain which also detects iron), (3) neurofilament immunohistochemistry (to estimate total neuronal number per explant) and (4) Alz-50 immunohistochemistry (to detect possible conformational changes in...Continue Reading

Citations

Feb 14, 2012·Biological Trace Element Research·Xin WuZe-Yuan Deng
Nov 6, 2002·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Tameko KihiraTomoyoshi Kondo
Dec 22, 2007·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Daniel KrewskiVirginie Rondeau

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