Do anti-angiogenic VEGF (VEGFxxxb) isoforms exist? A cautionary tale.

PloS One
Sheila HarrisChryso Kanthou

Abstract

Splicing of the human vascular endothelial growth factor-A (VEGF-A) gene has been reported to generate angiogenic (VEGFxxx) and anti-angiogenic (VEGFxxxb) isoforms. Corresponding VEGFxxxb isoforms have also been reported in rat and mouse. We examined VEGFxxxb expression in mouse fibrosarcoma cell lines expressing all or individual VEGF isoforms (VEGF120, 164 or 188), grown in vitro and in vivo, and compared results with those from normal mouse and human tissues. Importantly, genetic construction of VEGF164 and VEGF188 expressing fibrosarcomas, in which exon 7 is fused to the conventional exon 8, precludes VEGFxxxb splicing from occurring. Thus, these two fibrosarcoma cell lines provided endogenous negative controls. Using RT-PCR we show that primers designed to simultaneously amplify VEGFxxx and VEGFxxxb isoforms amplified only VEGFxxx variants in both species. Moreover, only VEGFxxx species were generated when mouse podocytes were treated with TGFβ-1, a reported activator of VEGFxxxb splice selection in human podocytes. A VEGF164/120 heteroduplex species was identified as a PCR artefact, specifically in mouse. VEGFxxxb isoform-specific PCR did amplify putative VEGFxxxb species in mouse and human tissues, but unexpectedly also ...Continue Reading

References

Jan 1, 1995·Breast Cancer Research and Treatment·N Ferrara
Jun 1, 1997·The Journal of Clinical Investigation·J GloyH Pavenstädt
Apr 22, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M TomisawaM Nakamura
Oct 26, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Y H LeeM Nakamura
Feb 13, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y S NgP A D'Amore
Feb 6, 2002·The Journal of Clinical Investigation·Ingeborg StalmansPatricia A D'Amore
Apr 12, 2002·Biochemical and Biophysical Research Communications·Lin ZhangGeorge Coukos
Sep 5, 2002·International Journal of Cancer. Journal International Du Cancer·Sonja M S UthoffSusan Galandiuk
Nov 1, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Harold F Dvorak
May 25, 2005·The Journal of Cell Biology·Sunyoung LeeM Luisa Iruela-Arispe
Jan 5, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Suparna Bonthala WedamSandra M Swain
Aug 16, 2006·Cellular and Molecular Life Sciences : CMLS·S Cébe SuarezK Ballmer-Hofer
Sep 5, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marcin Kowanetz, Napoleone Ferrara
Oct 10, 2006·Cancer Letters·Michael R LadomeryDavid O Bates
Feb 3, 2007·Journal of the American Society of Nephrology : JASN·Valérie Anne SchumacherBrigitte Royer-Pokora
Mar 24, 2007·The Journal of Medical Investigation : JMI·Hideki TomimotoSaburo Sone
May 16, 2007·The Journal of Pathology·C A StatonN J Brown
Jun 28, 2007·British Journal of Cancer·R O Pritchard-JonesD O Bates
Sep 14, 2007·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·E Miller-Kasprzak, P P Jagodziński
Nov 23, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yan QiuSteven J Harper
Jul 4, 2008·Nature Reviews. Cancer·Lee M Ellis, Daniel J Hicklin
Jul 29, 2008·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Emma S RennelSteven J Harper
Jun 13, 2009·Future Oncology·Emma S RennelDavid O Bates
Nov 17, 2009·Biochemical Society Transactions·Yan QiuDavid O Bates

❮ Previous
Next ❯

Citations

Dec 18, 2013·Cytokine & Growth Factor Reviews·Prakash VempatiFeilim Mac Gabhann
Oct 30, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·David O BatesSteven J Harper
Mar 12, 2013·Cellular and Molecular Life Sciences : CMLS·Sophie ChauvetFanny Mann
Aug 26, 2014·Neurobiology of Disease·R P HulseL F Donaldson
Feb 8, 2014·Current Eye Research·Takayuki BabaShuichi Yamamoto
May 2, 2014·Advances in Chronic Kidney Disease·Frank C Brosius, Richard J Coward
Jun 21, 2014·Cell·Sandeepa M EswarappaPaul L Fox
Mar 7, 2014·Pharmacology & Therapeutics·Annelies BronckaersIvo Lambrichts
Mar 20, 2013·Biochimie·Sandy Giuliano, Gilles Pagès
Oct 19, 2012·Cell Adhesion & Migration·Maria Peiris-Pagès
May 24, 2013·Blood·Thomas J CunninghamColin G Miles
Aug 13, 2013·PloS One·David O BatesSteven J Harper
Oct 7, 2016·Frontiers in Cell and Developmental Biology·Hideki YamamotoRandall S Johnson
Apr 25, 2018·International Journal of Molecular Sciences·Chloe J PeachJeanette Woolard
Dec 2, 2017·CPT: Pharmacometrics & Systems Pharmacology·Lindsay E CleggFeilim Mac Gabhann
Dec 30, 2014·Journal of the American Society of Nephrology : JASN·Sebastian OlteanAndrew H J Salmon
Mar 22, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Teagan J WalterStacey S Huppert
Mar 15, 2015·Pharmacological Reviews·Judy R van BeijnumArjan W Griffioen
Jul 22, 2018·Development·Sinem KaramanKari Alitalo
Dec 9, 2020·Journal of Experimental & Clinical Cancer Research : CR·Anna Di MatteoClaudia Ghigna
Mar 1, 2021·Progress in Retinal and Eye Research·Akiyoshi UemuraThomas Langmann
Jul 11, 2013·The American Journal of Pathology·Nicholas Beazley-LongLucy F Donaldson
May 13, 2020·Trends in Endocrinology and Metabolism : TEM·Hugues DardenteDavid Simpson
Sep 8, 2021·Frontiers in Pharmacology·Claudio BucoloAlessia Pascale

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
FCS
electrophoresis
biopsy
transgenic

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Bladder Carcinoma In Situ

Bladder Carcinoma In Situ is a superficial bladder cancer that occurs on the surface layer of the bladder. Discover the latest research on this precancerous condition in this feed.

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

© 2021 Meta ULC. All rights reserved