PMID: 2478816Jan 1, 1989Paper

Do cultured vascular smooth muscle cells resemble those of the artery wall? If not, why not?

Journal of Cardiovascular Pharmacology
E StadlerG R Campbell

Abstract

Ultrastructural stereological techniques were used to quantitate the phenotype of vascular smooth muscle cells grown in primary culture on a plastic substrate (control), on and within matrices of collagen type I, and on collagen type III, collagen type IV, or basement membrane Matrigel. The volume fraction of myofilaments (Vv myo) of freshly isolated cells at day 0 was 54.2 +/- 2.0%. By day 5 in culture, the Vv myo of cells grown on plastic, on collagen type I, and collagen type III had decreased significantly to 14.2 +/- 2.1%, 16.5 +/- 2.4%, and 16.3 +/- 2.9%, respectively. In contrast, smooth muscle cells grown within matrices of collagen type I, on collagen type IV, or on basement membrane Matrigel had a Vv myo of 30.7 +/- 1.5%, 30.8 +/- 4.2%, and 32.1 +/- 3.1%, respectively. These findings demonstrate that the presence of extracellular matrix components prevents modulation of smooth muscle phenotype early in culture and suggests that the extracellular matrix synthesized and secreted by smooth muscle in the normal vessel wall contributes to the maintenance of their contractile functional state.

Citations

Feb 1, 1991·Clinical and Experimental Pharmacology & Physiology·J H Campbell, G R Campbell
Dec 28, 1999·Tissue Engineering·T Kobashi, T Matsuda
Apr 28, 2006·Yonsei Medical Journal·Youngho LeeDucksun Ahn
Jan 1, 1990·Annals of the New York Academy of Sciences·J H CampbellG R Campbell
Dec 29, 2000·American Journal of Veterinary Research·D H RodgersonD L Kroll

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.