Do Cupins Have a Function Beyond Being Seed Storage Proteins?

Frontiers in Plant Science
Daša GábrišováMartin Hajduch

Abstract

Plants continue to flourish around the site of the Chernobyl Nuclear Power Plant disaster. The ability of plants to transcend the radio-contaminated environment was not anticipated and is not well understood. The aim of this study was to evaluate the proteome of flax (Linum usitatissimum L.) during seed filling by plants grown for a third generation near Chernobyl. For this purpose, seeds were harvested at 2, 4, and 6 weeks after flowering and at maturity, from plants grown in either non-radioactive or radio-contaminated experimental fields. Total proteins were extracted and the two-dimensional gel electrophoresis (2-DE) patterns analyzed. This approach established paired abundance profiles for 130 2-DE spots, e.g., profiles for the same spot across seed filling in non-radioactive and radio-contaminated experimental fields. Based on Analysis of Variance (ANOVA) followed by sequential Bonferroni correction, eight of the paired abundance profiles were discordant. Results from tandem mass spectrometry show that four 2-DE spots are discordant because they contain fragments of the cupin superfamily-proteins. Most of the fragments were derived from the N-terminal half of native cupins. Revisiting previously published data, it was fou...Continue Reading

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Citations

Jul 15, 2017·Molecular & Cellular Proteomics : MCP·Malika ChabiSimon Hawkins
Dec 21, 2018·International Journal of Radiation Biology·Maryna V KryvokhyzhaNamik M Rashydov
Jul 13, 2018·Frontiers in Plant Science·Nicol Caplin, Neil Willey

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

ImageMaster 2D Platinum
MassLynx
Protein Lynx Global Server

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