PMID: 22359918Mar 1, 2012Paper

Do electrostatic interactions determine glycation of hyaluronidase derivatives with N-acethylhexosamines?

Biomedit︠s︡inskai︠a︡ khimii︠a︡
A D TurashevA V Maksimenko

Abstract

Glycation of native hyaluronidase and its chondroitin sulfate modified form was studied with N-acethylglucosamine, N-acethylgalactosamine and their mixture, as well as hyaluronan fragments (n = 0-4) and their mixture. The modified form of hyaluronidase exhibited higher inactivation than native enzyme. The chondroitin sulfate modification of hyaluronidase altered its surface electrostatic potential, but this effect was not crucial for inactivation of hyaluronidase derivatives. The observed picture of the glycation action on hyaluronidase derivatives was opposite for glycation with mono- and di-saccharides. Such results give us the informative enzyme test for in vivo system in order to determine the dominant type of glycation agents in bloodstream and its origin.

References

Aug 1, 1992·Glycoconjugate Journal·K TakagakiM Endo
Jan 1, 1989·Annual Review of Biochemistry·J Lechner, F Wieland
Sep 15, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·A PollakG Lubec
Jun 14, 2001·Biochemistry. Biokhimii︠a︡·A V MaksimenkoE G Tischenko
Oct 22, 2004·Glycobiology·Nicholas T SeyfriedAndrew Almond
Aug 16, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Junbo GeHaozhu Chen
Mar 1, 2006·Circulation·Loredana G BucciarelliRavichandran Ramasamy
Feb 28, 2007·American Journal of Hypertension·Marie McNultyJohn Feely
Aug 25, 2007·Biotechnology Advances·Robert SternLadislav Soltés
May 1, 2008·The Journal of Biological Chemistry·Paul A GrimsrudDavid A Bernlohr
Nov 18, 2008·Glycoconjugate Journal·Fengchao ChenMasahiko Endo

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.