Do Induced Pluripotent Stem Cell Characteristics Correlate with Efficient In Vitro Smooth Muscle Cell Differentiation? A Comparison of Three Patient-Derived Induced Pluripotent Stem Cell Lines

Stem Cells and Development
Yingying ZhouBertha Chen

Abstract

Human induced pluripotent stem cells (iPSCs) have the potential to repair/regenerate smooth muscle cells (SMCs) in different organs. However, there are many challenges in their translation to clinical therapies. In this study, we describe our observations of in vitro SMC differentiation in three iPSC lines derived from human fibroblasts using retroviral, episomal, and mRNA/miRNA reprogramming methods. We sought to elucidate correlations between differentiation characteristics and efficiencies that can facilitate large-scale production of differentiated cells for clinical applications, and to report differences in pluripotency marker expression in differentiated cells from different iPSC lines. A standardized SMC differentiation protocol was used to induce the CD31+/CD34+ vascular progenitor cell phenotype. These were sorted by magnetic-activated (MACS) and fluorescence-activated cell sorting (FACS), and then treated with PDGF-BB and smooth muscle growth medium for further differentiation into smooth muscle progenitor cells (pSMCs). The expression of SMC and pluripotency markers in early- and late-passage (P1 and P4) pSMCs was analyzed. A total of 36 differentiation runs was performed on the three patient iPSC lines. All pSMC po...Continue Reading

References

Oct 30, 1971·Lancet·M Seabright
Jul 1, 1996·The Journal of Cell Biology·F T van der LoopG J van Eys
May 4, 2002·Nature Reviews. Molecular Cell Biology·James J TomasekRobert A Brown
Sep 13, 2005·Cell·Laurie A BoyerRichard A Young
Nov 16, 2005·Gastroenterology·Petra NiessenGuillaume van Eys
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Cheng-Nan ChenJeng-Jiann Chiu
Nov 22, 2007·Science·Junying YuJames A Thomson
Sep 28, 2010·Nucleic Acids Research·Shi-Lung LinDavid T S Wu
Apr 9, 2011·Cell Stem Cell·Frederick Anokye-DansoEdward E Morrisey
May 31, 2011·Cell Stem Cell·Norikatsu MiyoshiMasaki Mori
Dec 7, 2013·Stem Cells Translational Medicine·Melanie MarchandRenee A Reijo Pera
Jun 10, 2017·Circulation Research·Yoshinori Yoshida, Shinya Yamanaka
Sep 2, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Eithne Margaret MaguireQingbo Xu

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Methods Mentioned

BETA
magnetic-activated cell sorting
transfections
transfection
PCR
FACS
fluorescence-activated cell sorting
SMA

Software Mentioned

WiCell
MxPro QPCR
SPSS

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