Do mitochondria make nitric oxide? no?

Free Radical Research
Yvonne Mei Sian TayBarry Halliwell

Abstract

Several papers have claimed that mitochondria contain nitric oxide synthase (NOS) and make nitric oxide (NO*) in amounts sufficient to affect mitochondrial respiration. However, we found that the addition of L-arginine or the NOS inhibitor L-NMMA to intact rat liver mitochondria did not have any effect on the respiratory rate in both State 3 and State 4. We did not detect mitochondrial NO* production by the oxymyoglobin oxidation assay, or electrochemically using an NO* electrode. An apparent NO* production detected by the Griess assay was identified as an artifact. NO* generated by eNOS added to the mitochondria could easily be detected, although succinate-supplemented mitochondria appeared to consume NO*. Our data show that NO* production by normal rat liver mitochondria cannot be detected in our laboratory, even though the levels of production claimed in the literature should easily have been measured by the techniques used. The implications for the putative mitochondrial NOS are discussed.

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Citations

Apr 18, 2009·The Journal of Biological Chemistry·Priya VenkatakrishnanR Timothy Miller
Feb 4, 2009·Antioxidants & Redox Signaling·David F Stowe, Amadou K S Camara
Dec 18, 2012·International Journal of Molecular Sciences·Celia Harumi TenganRosely Oliveira Godinho
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Jan 15, 2015·Toxicological Research·Aekyong Kim
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