PMID: 11607049Jan 1, 1990Paper

Do thylakoids really contain phosphatidylcholine?

Proceedings of the National Academy of Sciences of the United States of America
A J DorneR Douce

Abstract

Isolated intact spinach chloroplasts were incubated with phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3) under mild experimental conditions in which only the phosphatidylcholine localized in the cytosolic leaflet of the outer envelope membrane can be hydrolyzed. Thylakoids, which were protected from phospholipase C degradation, were subsequently prepared from the phospholipase C-treated chloroplasts and found to be devoid of phosphatidylcholine. Previously reported occurrences of phosphatidylcholine in thylakoid preparations probably reflect contamination of the thylakoids by envelope membranes. In the present work, contamination of thylakoids by envelope membranes was determined by measuring the 1,2-diacylglycerol 3-beta-galactosyltransferase [monogalactosyldiacylglycerol (MGDG) synthase; UDPgalactose: 1,2-diacylglycerol 3-beta-D-galactosyltransferase, EC 2.4.1.46] in the different chloroplast subfractions. We conclude that phosphatidylcholine is not present in highly purified thylakoids. Phosphatidylcholine is also absent from prokaryotic cyanobacterial membranes, and our results are in agreement with the endosymbiotic origin of higher plant chloroplasts.

References

May 1, 1985·The Journal of Cell Biology·A J DorneR Douce
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·S W JeffreyA A Benson
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K ClineK Keegstra

❮ Previous
Next ❯

Citations

Jul 20, 2007·Photosynthesis Research·Hajime Wada, Norio Murata
Apr 21, 1999·Progress in Lipid Research·P MoreauC Cassagne
May 29, 2002·Journal of Photochemistry and Photobiology. B, Biology·Xiang RuanNanming Zhao
Aug 1, 1991·European Journal of Biochemistry·J JoyardR Douce
Jul 4, 2009·Annual Review of Cell and Developmental Biology·Christoph Benning
Sep 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·H HärtelC Benning
Mar 13, 2014·Plant & Cell Physiology·Yasusi YamamotoYasuo Ishikawa
May 9, 2012·The Journal of Cell Biology·Jose M Celedon, Kenneth Cline
Nov 3, 2009·Progress in Lipid Research·Jacques JoyardNorbert Rolland
Apr 29, 2008·Progress in Lipid Research·Christoph Benning
Sep 21, 2013·Biochimica Et Biophysica Acta·Laurence BoudièreEric Maréchal
May 17, 2011·Biochimica Et Biophysica Acta·Naoki Mizusawa, Hajime Wada
Apr 14, 2015·Current Opinion in Cell Biology·Maryse A Block, Juliette Jouhet
Mar 28, 2016·Plant & Cell Physiology·Miho Yoshioka-Nishimura
Oct 26, 2016·Biochimica Et Biophysica Acta. General Subjects·Pavel Pospíšil, Yasusi Yamamoto
Dec 7, 2017·Frontiers in Plant Science·Koichi KobayashiHajime Wada
Mar 27, 2019·Biological Chemistry·Anne-Christin Pohland, Dirk Schneider
Dec 13, 2006·The Journal of Biological Chemistry·Markus FritzErnst Heinz
Dec 27, 2019·Journal of Phycology·María José IglesiasFernando López Ortiz
Jun 21, 2019·Scientific Reports·Jasmine TheisMichael Schroda
Oct 28, 2019·The Biochemical Journal·Petar H Lambrev, Parveen Akhtar
Jul 19, 2018·Frontiers in Plant Science·Yue Niu, Yun Xiang
Apr 23, 2020·Proceedings of the National Academy of Sciences of the United States of America·Michael Schroda
Jul 2, 2018·Photosynthesis Research·Evan LaBrantRebecca L Roston
Jun 2, 2000·Biochemical and Biophysical Research Communications·D Seigneurin-BernyJ Joyard

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.