Do transplanted corneal limbal stem cells survive in vivo long-term? Possible techniques to detect donor cell survival by polymerase chain reaction with the amelogenin gene and Y-specific probes

Eye
T R HendersonB A Noble

Abstract

To investigate donor cell survival following corneal limbal stem cell grafting, which is based on the corneal stem cell model. We describe the use of the amelogenin gene probe with the polymerase chain reaction (PCR) to detect surviving donor cells and report preliminary studies using Y-specific DNA probes. DNA polymorphisms have a detection limit of 10%. The SRY 'Y-specific' probe has a theoretical detection limit of 1 cell in 10,000. The techniques were applied to investigate survival of male donor cells in an aniridic female patient 2 1/2 years following limbal stem cell grafts. We speculate that low levels of donor-derived cells may still be present. We discuss the advantages and disadvantages of the two approaches, which may have future clinical and experimental application.

References

Jan 1, 1979·International Ophthalmology Clinics·R A Thoft
Feb 1, 1990·Archives of Ophthalmology·P W TurgeonR A Thoft
May 1, 1989·Ophthalmology·K R Kenyon, S C Tseng
Nov 1, 1985·Archives of Ophthalmology·R A WeiseA M Roth
Sep 1, 1974·American Journal of Ophthalmology·J S Haas, R H Nootens
Sep 1, 1995·American Journal of Ophthalmology·K NishidaS Yamamoto
Aug 1, 1946·Archives of Ophthalmology·W F HUGHES

❮ Previous
Next ❯

Citations

Aug 9, 2002·DNA and Cell Biology·J E MooreA P Adamis
May 24, 2007·Tissue Engineering·Justin R SharpeS Elizabeth James
Apr 24, 2001·The British Journal of Ophthalmology·T R HendersonK A Williams
Jan 15, 2014·Optometry and Vision Science : Official Publication of the American Academy of Optometry·David P Roncone
May 1, 2007·Regenerative Medicine·Sajjad AhmadMajlinda Lako
Aug 2, 2006·Experimental Eye Research·S KeijserM J Jager
Apr 8, 2004·Eye·E M EspanaS C G Tseng
Sep 2, 2003·European Journal of Ophthalmology·K Ramaesh, B Dhillon
Feb 13, 2021·Genesis : the Journal of Genetics and Development·Mohd Tayyab Adil, Jonathan J Henry

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.