Do unsaturated phosphoinositides mix with ordered phosphadidylcholine model membranes?

Journal of Lipid Research
Antje Hermelink, G Brezesinski

Abstract

Phosphoinositides have been shown to control membrane trafficking events by targeting proteins to specific cellular sites, which requires a tight regulation of phosphoinositide generation and turnover as well as a high degree of compartmentalization. To shed light on the processes that lead to the formation of phosphoinositide-enriched microdomains, mixed monolayers of phosphatidylcholine and dioleoyl-phosphatidylinositol (DOPtdIns) or dioleoyl-phosphatidylinositol-bisphosphate [DOPtdIns(4,5)P(2)] were investigated by isothermal area/pressure measurements, Brewster angle microscopy, and grazing incidence X-ray diffraction. The results are consistent with a charge-dependent formation of phosphatidylinositol-containing tightly packed phases. DOPtdIns is capable of mixing partially with condensed 1,2-distearoyl-phosphatidylcholine (DSPC) and of forming mixed crystals that differ significantly from those formed by pure DSPC. DOPtdIns(4,5)P(2) in mixtures with DSPC is, to a much larger extent, phase separated. The observed phase separation of the highly charged DOPtdIns(4,5)P(2) is presumably water stabilized by electrostatic interactions and hydrogen bonding. In biological systems, an enzymatic phosphorylation of phosphatidylinosit...Continue Reading

References

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Citations

Feb 22, 2012·Progress in Lipid Research·Peter J Quinn
Jun 4, 2011·Chemistry and Physics of Lipids·Franco Vega MercadoNatalia Wilke
Nov 28, 2009·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Hélder A SantosCarlos M Pereira
Mar 27, 2010·Biophysical Journal·Dmitry LupyanRoman Osman
Aug 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Emanuel SchneckJean Daillant
Apr 2, 2020·International Journal of Molecular Sciences·Nirupama DevanathanAnn C Kimble-Hill
Feb 7, 2009·The Biochemical Journal·Dominic Poccia, Banafshé Larijani
Jul 15, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Siwar TrabelsiDaniel K Schwartz
Oct 13, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Victoria M LatzaEmanuel Schneck
Oct 19, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Samantha MicciullaEmanuel Schneck
Oct 26, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Victoria M LatzaEmanuel Schneck

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