Docosahexaenoic acid antagonizes the boosting effect of palmitic acid on LPS inflammatory signaling by inhibiting gene transcription and ceramide synthesis

PloS One
Junfei JinYan Huang

Abstract

It is well known that saturated fatty acids (SFAs) and unsaturated fatty acid, in particular omega-3 polyunsaturated fatty acids (n-3 PUFAs), have different effects on inflammatory signaling: SFAs are pro-inflammatory but n-3 PUFAs have strong anti-inflammatory properties. We have reported that palmitic acid (PA), a saturated fatty acid, robustly amplifies lipopolysaccharide (LPS) signaling to upregulate proinflammatory gene expression in macrophages. We also reported that the increased production of ceramide (CER) via sphingomyelin (SM) hydrolysis and CER de novo synthesis plays a key role in the synergistic effect of LPS and PA on proinflammatory gene expression. However, it remains unclear if n-3 PUFAs are capable of antagonizing the synergistic effect of LPS and PA on gene expression and CER production. In this study, we employed the above macrophage culture system and lipidomical analysis to assess the effect of n-3 PUFAs on proinflammatory gene expression and CER production stimulated by LPS and PA. Results showed that DHA strongly inhibited the synergistic effect of LPS and PA on proinflammatory gene expression by targeting nuclear factor kappa B (NFκB)-dependent gene transcription. Results also showed that DHA inhibited...Continue Reading

References

Sep 6, 2002·Biochemical Pharmacology·Linda VermeulenGuy Haegeman
Dec 14, 2004·The Journal of Biological Chemistry·Mohammad Reza HojjatiXian-Cheng Jiang
Jun 4, 2005·Cell Death and Differentiation·F DemarchiC Schneider
Mar 15, 2006·Apoptosis : an International Journal on Programmed Cell Death·J StibanM Colombini
Jan 15, 2008·Cellular Signalling·Kazuyuki KitataniYusuf A Hannun
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Yusuf A Hannun, Lina M Obeid
Apr 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kevin N CouperEleanor M Riley
Nov 20, 2008·Journal of Lipid Research·Nana Bartke, Yusuf A Hannun
Dec 9, 2008·Antioxidants & Redox Signaling·Milton HamblinY Eugene Chen
Jan 30, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Eric A SchwartzPeter D Reaven
Jun 15, 2010·Current Molecular Medicine·Y H Zeidan, Y A Hannun
Dec 21, 2010·Prostaglandins & Other Lipid Mediators·Zhi-Qiang ChangTae-Sik Park
Jan 27, 2011·American Journal of Physiology. Endocrinology and Metabolism·Christopher D Green, L Karl Olson
Oct 18, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hai-Yan QiJames H Shelhamer
Jan 18, 2012·The Biochemical Journal·Thomas D MullenLina M Obeid
Sep 22, 2012·Trends in Cell Biology·Laura TornatoreGuido Franzoso
Dec 20, 2012·The Journal of Biological Chemistry·Joel D SchillingJean E Schaffer
Jun 21, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jason HellmannMatthew Spite
Oct 23, 2016·Trends in Endocrinology and Metabolism : TEM·Linda SassetAnnarita Di Lorenzo
Oct 4, 2016·The Journal of Nutritional Biochemistry·Begoña Ruiz-NúñezFrits A J Muskiet
Feb 12, 2017·Metabolism: Clinical and Experimental·Júnia Maria Geraldo GomesRita de Cássia Gonçalves Alfenas

❮ Previous
Next ❯

Citations

Jul 16, 2019·Journal of Dairy Science·J W McFadden, J E Rico
Aug 15, 2020·The British Journal of Nutrition·Chelsey WalchukMiyoung Suh
Jul 4, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Peng LiJianbin He
Oct 17, 2019·International Journal of Molecular Sciences·Saray GutiérrezMaria E Johansson
Mar 31, 2020·Molecular and Cellular Biochemistry·Raimundo Fernandes de Araujo JuniorLuis J Cruz
Jul 7, 2019·Journal of Clinical Medicine·Sarah BazarganipourSabine Grösch
Aug 1, 2019·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Jan Korbecki, Karolina Bajdak-Rusinek
Aug 6, 2020·Biomolecules·Viktoriya S ShramkoYuliya I Ragino
Jul 13, 2021·Frontiers in Cardiovascular Medicine·Nathalie DonisCécile Oury
Jul 25, 2021·International Journal of Molecular Sciences·Stanislav Kotlyarov, Anna Kotlyarova

❮ Previous
Next ❯

Methods Mentioned

BETA
gel filtration
PCR
transfection
Assay
ELISA
nuclear translocation

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here