PMID: 9630720Jun 19, 1998Paper

Docosahexaenoic acid (DHA) alters the structure and composition of membranous vesicles exfoliated from the surface of a murine leukemia cell line

Biochimica Et Biophysica Acta
E E WilliamsW Stillwell

Abstract

Membrane lipid microdomains are regions of the membrane thought to be functionally important, but which have remained poorly characterized because they have proven to be difficult to isolate. The exfoliation of small membranous vesicles from the cell surface is a continuous and normal activity in many cells. If microdomains are relatively large or stable, they may influence the structure and composition of exfoliated vesicles, which are easy to isolate. We tested the ability of docosahexaenoic acid (DHA), a fatty acid proposed to alter the structure of microdomains, to change the structure and composition of vesicles exfoliated from a murine leukemia cell line. Cells were cultured in normal and DHA-enriched media for 72 h, then washed and given a 15-h exfoliation period. Afterwards, the pooled vesicles and their parent plasma membrane were collected and analyzed. Vesicles and plasma membrane from cells grown in normal culture medium had similar fatty acid compositions, including equal, and low, proportions of DHA, but the vesicles had much more cholesterol and displayed higher anisotropy than the plasma membrane. When cells were grown in DHA-enriched medium, both the plasma membrane and exfoliated vesicles had 10-fold elevated ...Continue Reading

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Citations

Jun 1, 2005·Progress in Lipid Research·Arlene D Albert, Kathleen Boesze-Battaglia
Oct 29, 2003·Chemistry and Physics of Lipids·William Stillwell, Stephen R Wassall
Sep 20, 2005·Breast Cancer Research : BCR·Rafat A SiddiquiWilliam Stillwell
Jul 14, 2011·Biochemical and Biophysical Research Communications·Jeffrey D AltenburgRafat A Siddiqui
May 25, 2016·Chemistry and Physics of Lipids·Arlene AlbertKathleen Boesze-Battaglia
Oct 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Carla C C R de Carvalho, Maria José Caramujo
Feb 29, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Reijo KäkeläMatti Vornanen
Apr 9, 2005·The American Journal of Clinical Nutrition·Eileen E BirchDennis R Hoffman
Nov 8, 2020·Archives of Biochemistry and Biophysics·Tayana Mazin TsuboneMauricio S Baptista

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