Docosahexaenoic acid prevents dendritic cell maturation and in vitro and in vivo expression of the IL-12 cytokine family.

Lipids in Health and Disease
Weimin KongDoina Ganea

Abstract

Acute and chronic inflammation play essential roles in inflammatory/autoimmune conditions. Protective anti-inflammatory effects of the n-3 fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were reported in animal models of colitis, sepsis, and stroke. Since dendritic cells (DC) represent the essential cellular link between innate and adaptive immunity and have a prominent role in tolerance for self-antigens, we sought to investigate the impact of DHA on DC maturation and proinflammatory cytokine production. Murine bone marrow-derived DC were treated with DHA and stimulated with various toll-like receptor (TLR) ligands. Flow cytometry was used to determine the levels of surface maturation markers and endocytic activity. Cytokine expression and secretion were measured by real-time RT-PCR and ELISA assays. PPARgamma and NFkappaB activity in nuclear extracts were determined by binding to specific oligonucleotide sequences using ELISA-based assays. In vivo effects of DHA were assessed in splenic DC from LPS-inoculated mice maintained on a DHA-enriched diet. DHA maintained the immature phenotype in bone marrow-derived DC by preventing the upregulation of MHCII and costimulatory molecules (CD40, CD80 and CD86) and...Continue Reading

References

Sep 5, 2001·Clinical and Experimental Immunology·J L ArringtonD N McMurray
Aug 19, 2003·The Journal of Biological Chemistry·Victor L MarcheselliNicolas G Bazan
Jan 7, 2004·International Archives of Allergy and Immunology·Thomas M Stulnig
Feb 3, 2005·The Journal of Biological Chemistry·Maximilian ZeydaThomas M Stulnig
Apr 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amy R WeatherillDaniel H Hwang
Oct 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christine E LoscherHelen M Roche
May 16, 2006·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·D CamuescoJ Gálvez
Jul 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Christian A HudertJing X Kang
Aug 8, 2006·Biotechnology Journal·Undurti N Das
Mar 6, 2007·Trends in Immunology·Amiram Ariel, Charles N Serhan
Mar 23, 2007·Intensive Care Medicine·Thomas-Thi PluessRené L Chioléro
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amir F SheibanieDoina Ganea
Nov 6, 2007·Trends in Immunology·Daniel S Straus, Christopher K Glass
Feb 1, 2008·Annual Review of Pathology·Charles N SerhanRong Yang
Apr 26, 2008·Nature Reviews. Immunology·Charles N SerhanThomas E Van Dyke
Oct 18, 2008·Immunobiology·Attila Szanto, Laszlo Nagy
Oct 28, 2008·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Philip C Calder
Nov 15, 2008·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Charles N Serhan
Dec 4, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kie KasugaCharles N Serhan
Feb 17, 2009·Human Immunology·Kerstin SteinbrinkHelmut Jonuleit
Mar 17, 2009·Journal of Dermatological Science·Sayuri Yamazaki, Ralph M Steinman
May 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amit AwasthiMohamed Oukka
Jun 9, 2009·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Robert S ChapkinDavid N McMurray
May 6, 2014·The Journal of Nutritional Biochemistry·Eve DraperChristine E Loscher

❮ Previous
Next ❯

Citations

Mar 1, 2012·The Proceedings of the Nutrition Society·Philip C Calder
May 15, 2013·The Proceedings of the Nutrition Society·Philip C Calder
Sep 2, 2010·Cell Research·Philip C Calder
Apr 19, 2013·Current Opinion in Lipidology·Chuchun L Chang, Richard J Deckelbaum
Mar 1, 2012·World Journal of Gastroenterology : WJG·Sarah J McCaskeyJenifer I Fenton
Apr 3, 2014·Cancer Immunology, Immunotherapy : CII·Sheng XiaLing Qi
Aug 6, 2011·European Journal of Pharmacology·Philip C Calder
Dec 30, 2015·PPAR Research·Amanda CroasdellRichard P Phipps
Sep 30, 2015·Progress in Lipid Research·Julie Boisramé-HelmsFerhat Meziani
Jan 3, 2013·Journal of Paediatrics and Child Health·Kerryn AtwellAndrew J McPhee
Mar 26, 2016·Life Sciences·Bianca W P LoboGisela Maria Dellamora-Ortiz
Sep 1, 2015·Neurochemistry International·Kusumika GharamiSumantra Das
Jul 27, 2011·Vaccine·Jose Rey-LadinoRonald Quinn
May 24, 2016·European Journal of Immunology·Kristin StelznerAnja Saalbach
Oct 30, 2016·The Journal of Infection·Marie-Odile HussonFrédéric Gottrand
Jun 1, 2017·Brain, Behavior, and Immunity·Yosuke OhtakeShuxin Li
Jan 27, 2012·The Journal of Nutrition·Philip C Calder
Aug 11, 2017·Cellular and Molecular Neurobiology·Glaiza A TanAdil J Nazarali
Feb 12, 2015·Beilstein Journal of Organic Chemistry·Éva RajnavölgyiÉva Fenyvesi
Mar 17, 2020·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Shoresh BarkhordariRoya Kelishadi
Aug 12, 2020·Molecular Nutrition & Food Research·Paulina WawrzyniakMartin Hersberger
Jul 7, 2020·The Proceedings of the Nutrition Society·Philip C Calder
May 10, 2013·Current Opinion in Clinical Nutrition and Metabolic Care·Philip C Calder
Jul 5, 2017·Current Treatment Options in Psychiatry·Jennifer K MelbourneRajiv P Sharma
Aug 16, 2018·The Journal of Clinical Investigation·Parker F DuffneyPatricia J Sime
Dec 11, 2019·Nutrients·Urszula RadzikowskaMilena Sokolowska
Oct 17, 2019·International Journal of Molecular Sciences·Saray GutiérrezMaria E Johansson
Sep 26, 2020·Frontiers in Pharmacology·Astrid HogenkampLinette E M Willemsen
May 8, 2020·Science Translational Medicine·Byron BrookNelly Amenyogbe
Jul 7, 2012·British Journal of Clinical Pharmacology·Philip C Calder
Sep 14, 2017·Biochemical Society Transactions·Philip C Calder

❮ Previous
Next ❯

Methods Mentioned

BETA
nuclear translocation
ELISA
transcription factor assay
FACS
flow cytometry
PCR
Infrared Imaging
transgenic

Software Mentioned

Cellquest

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.