Docosahexaenoic acid promotes micron scale liquid-ordered domains. A comparison study of docosahexaenoic versus oleic acid containing phosphatidylcholine in raft-like mixtures

Biochimica Et Biophysica Acta
R GeorgievaGalya Staneva

Abstract

The understanding of the functional role of the lipid diversity in biological membranes is a major challenge. Lipid models have been developed to address this issue by using lipid mixtures generating liquid-ordered (Lo)/liquid-disordered (Ld) immiscibility. The present study examined mixtures comprising Egg sphingomyelin (SM), cholesterol (chol) and phosphatidylcholine (PC) either containing docosahexaenoic (PDPC) or oleic acid (POPC). The mixtures were examined in terms of their capability to induce phase separation at the micron- and nano-scales. Fluorescence microscopy, electron spin resonance (ESR), X-ray diffraction (XRD) and calorimetry methods were used to analyze the lateral organization of the mixtures. Fluorescence microscopy of giant vesicles could show that the temperature of the micron-scale Lo/Ld miscibility is higher for PDPC than for POPC ternary mixtures. At 37°C, no micron-scale Lo/Ld phase separation could be identified in the POPC containing mixtures while it was evident for PDPC. In contrast, a phase separation was distinguished for both PC mixtures by ESR and XRD, indicative that PDPC and POPC mixtures differed in micron vs nano domain organization. Compared to POPC, the higher line tension of the Lo domai...Continue Reading

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Citations

Apr 28, 2016·Biophysical Journal·Kandice R LeventalIlya Levental
Aug 11, 2016·Biophysical Journal·Oskar EngbergThomas K M Nyholm
May 12, 2017·Physical Chemistry Chemical Physics : PCCP·Naofumi ShimokawaMasahiro Takagi
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Apr 23, 2021·Biophysical Journal·Miranda L JacobsNeha P Kamat
Sep 19, 2020·Chemistry and Physics of Lipids·Jacob J KinnunJohn Katsaras

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