Docosapentaenoic acid (22:5,n-3): metabolism and effect on prostacyclin production in endothelial cells

Prostaglandins, Leukotrienes, and Essential Fatty Acids
C BénistantM Lagarde

Abstract

Eicosapentaenoic acid (EPA, 20:5,n-3) and docosahexaenoic acid (DHA, 22:6, n-3), the two main fatty acids of fish oil, have been shown to inhibit prostacyclin production and to be actively interconverted, leading to the accumulation of docosapentaenoic acid (DPA, 22:5,n-3) in endothelial cell phospholipids. We have investigated the effect of supplementing endothelial cells with DPA on their capacity to produce prostacyclin. We found that endothelial cells incubated for 22 h with 25 microM DPA bound to albumin (fatty acid/albumin ratio of 1.3) produced two-fold less prostacyclin compared to control cells when stimulated with endogenous arachidonic acid-mobilizing agents such as bradykinin and calcium ionophore A23187. Since the formation of prostacyclin from 0.1-15 microM exogenous arachidonic acid was also reduced, it is suggested that prostacyclin inhibition observed in DPA-treated cells might not proceed from a reduction of arachidonic acid availability only. Such an inhibition was already observed after 1 h incubation of the cells with DPA, and with 2-20 times lower DPA concentrations. The inhibition might depend on EPA which was formed by retroconversion of DPA.

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Citations

May 21, 2010·The Journal of Nutrition·S Coosje DijkstraDavid B Thomas
Jun 15, 2013·Canadian Urological Association Journal = Journal De L'Association Des Urologues Du Canada·Michael E ChuaMarcelino L Morales
Apr 17, 2013·Prostate Cancer·Mishell Kris Sorongon-LegaspiMarcelino Morales
Aug 3, 2011·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Gunveen KaurNicky Konstantopoulos
Aug 13, 2013·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Caroline MorinSamuel Fortin
Jul 27, 2010·Progress in Lipid Research·Gunveen KaurAndrew J Sinclair
Sep 30, 2014·Biochimica Et Biophysica Acta·Michel GuichardantEvelyne Véricel
Jul 11, 2008·The American Journal of Clinical Nutrition·Qi SunFrank B Hu
Jun 13, 2017·Prostaglandins & Other Lipid Mediators·Trond Vidar HansenCharles N Serhan

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