Does cellular sex matter? Dimorphic transcriptional differences between female and male endothelial cells

Atherosclerosis
Mario LorenzVerena Stangl

Abstract

Significant sex differences exist in cardiovascular diseases. Although an impact of gonadal hormones is presumed, it is largely unknown whether sexually dimorphic gene expression also plays a role and whether cells themselves show intrinsic sex differences. We performed whole genome expression analyses in human umbilical vein endothelial cells (HUVEC) from 20 male and 20 female donors and compared levels of gene transcription between the sexes. To further assess whether there is a sex-specific response to stress, we subjected male and female HUVEC to shear for 24 h and analysed changes in gene expression. Genes indicative for greater immune responsiveness were stronger expressed in female compared to male HUVEC. There was a significant enrichment of 77 immune-related genes in female HUVEC. These increased transcriptional levels in female cells were verified for 20 genes by real-time RT-PCR. 6.7% of all mRNAs were regulated by shear stress. Female HUVEC showed a more pronounced transcriptional response to shear than did their male counterparts. In addition to quantitative differences, a number of genes were regulated in the opposite direction between the two sexes by shear stress. Functionally, female HUVEC showed a higher cell ...Continue Reading

References

Mar 10, 2001·Cell·C K Glass, J L Witztum
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·S M McCormickK K Chittur
Oct 30, 2003·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Marquis P VawterWilliam E Bunney
Dec 2, 2004·Nature Reviews. Genetics·Roger HortonStephan Beck
Apr 9, 2005·Human Reproduction Update·Annechien BoumanMarijke M Faas
Jul 6, 2005·European Heart Journal·Nicoline JochmannVerena Stangl
Jul 11, 2006·Genome Research·Xia YangAldons J Lusis
Jan 31, 2007·Journal of Autoimmunity·Norbert Gleicher, David H Barad
Jul 25, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jörg IsenseePatricia Ruiz Noppinger
Feb 15, 2008·European Heart Journal·Verena StanglKarl Stangl
Oct 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Nicole C BerchtoldCarl W Cotman
Nov 4, 2008·BMC Evolutionary Biology·Reinmar HagerJason B Wolf
Nov 28, 2008·The Journal of Biological Chemistry·Lina DuRobert S B Clark
Dec 17, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Angela MaselliWalter Malorni
Oct 7, 2009·FEBS Letters·Elisabetta StrafaceFlavia Franconi
Feb 9, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Jianjie WangVirginia H Huxley
Apr 20, 2010·Annals of the New York Academy of Sciences·Maurizio CutoloPier Carlo Sarzi Puttini
Jul 10, 2010·Science·Christopher GreggCatherine Dulac
Mar 3, 2011·Journal of Cellular and Molecular Medicine·Pasquale ListaWalter Malorni
Apr 1, 2011·Current Pharmaceutical Design·Marina PierdominiciWalter Malorni
Oct 18, 2011·Vascular Medicine·Blair D JohnsonJanet P Wallace
Nov 17, 2011·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·J A BarryM Thomas
Nov 22, 2011·Autoimmunity Reviews·Udi Nussinovitch, Yehuda Shoenfeld
Dec 14, 2011·Autoimmunity Reviews·Sabine Oertelt-Prigione
Dec 15, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Yingfang TianFrank R Sharp
Feb 3, 2012·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Masahiro Akishita, Jing Yu
Mar 22, 2012·Endocrinology·Arthur P Arnold, Aldons J Lusis
Apr 10, 2012·Pharmacology & Therapeutics·A A Knowlton, A R Lee
Aug 7, 2012·Current Heart Failure Reports·Shannon M Dunlay, Véronique L Roger
Oct 3, 2012·Handbook of Experimental Pharmacology·Elisabetta StrafaceWalter Malorni
Oct 20, 2012·Nucleic Acids Research·James RobinsonSteven G E Marsh
Nov 28, 2012·Hypertension·Vera Regitz-ZagrosekDuska Dragun
May 2, 2013·Physiological Genomics·Alicia M EvangelistaElizabeth Murphy
Aug 2, 2013·Nature·Elizabeth Pollitzer
Sep 24, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stacey A RitzDonna Mergler

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Citations

Oct 21, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Ilaria CampesiFlavia Franconi
Jan 22, 2017·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Neja MudrovcicKarolina Kublickiene
Jun 10, 2018·The Journal of Physiology·Virginia H HuxleyJianjie Wang
Aug 11, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Yuhao ZhangKrithika Lingappan
Aug 2, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Thomas P KeeleyGiovanni E Mann
Jul 25, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andreas BennPetra Knaus
Nov 4, 2016·Physiological Reviews·Vera Regitz-Zagrosek, Georgios Kararigas
Aug 31, 2017·Scientific Reports·Maria Grazia CattaneoLucia Maria Vicentini
May 9, 2019·Respirology : Official Journal of the Asian Pacific Society of Respirology·Clara E Green
Oct 13, 2020·Advanced Healthcare Materials·Bryan D JamesJosephine B Allen
Jan 17, 2021·Life·Ilaria CampesiGiampiero Capobianco
Aug 6, 2020·Journal of Vascular Surgery. Vascular Science·Tambudzai KudzeAlan Dardik
Jan 9, 2021·Biology of Sex Differences·Maria Grazia CattaneoLucia M Vicentini
Jun 2, 2021·Molecular and Cellular Endocrinology·Michaela Keuper, Martin Jastroch
Jun 19, 2021·Advanced Healthcare Materials·Bryan D James, Josephine B Allen
Jul 20, 2021·Journal of the American Heart Association·Cheryl M J TanMariane Bertagnolli

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