Does cholesterol discriminate between sphingomyelin and phosphatidylcholine in mixed monolayers containing both phospholipids?

Chemistry and Physics of Lipids
P Mattjus, J P Slotte

Abstract

The objective of this work was to examine the interaction of cholesterol with both phosphatidylcholines, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) or 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and sphingomyelins, N-oleoyl-D-sphingomyelin (O-SPM) or N-palmitoyl-D-sphingomyelin (P-SPM), in monolayers at an air/water interface. We used cholesterol oxidase to probe for the relative strength of sterol-phospholipid interaction, and fluorescence microscopy to visualize lateral domain formation in the mixed monolayers. The ternary mixed monolayers, which contained cholesterol, POPC, and O-SPM had a twofold higher average oxidation rate than the corresponding system containing DPPC and P-SPM. This difference in oxidation rate between saturated and unsaturated systems was observed irrespective of the ratio between phosphatidylcholine and sphingomyelin in the monolayer. With either the saturated or the unsaturated systems, however, the rate of oxidation was influenced by the ratio of phosphatidylcholine to sphingomyelin. As the monolayer content of phosphatidylcholine increased and the sphingomyelin content decreased correspondingly (to maintain a constant cholesterol-to-phospholipid molar ratio), an increase in the...Continue Reading

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