Does chondroitin sulfate have a role to play in the morphogenesis of the chick primary corneal stroma?

Developmental Biology
M K BansalJ B Bard

Abstract

This paper makes three points about how the chick corneal epithelium lays down the primary stroma, an orthogonally arranged array of well-spaced, 20-nm-diameter collagen fibrils. (1) Isolated corneal epithelia will, when cultured, lay down de novo stromas whose fibril-diameter distribution, fibril spacing, and proteoglycan profile are similar to those laid down in vivo. They differ from embryonic stromas in two ways: first, much of the chondroitin sulfate is released to the medium and, second, there is a relatively small amount of orthogonal organization. Epithelia seem only to lay down such stromas if they are separated from their original stromas with dispase, which leaves an intact basal lamina, and spread out, basal lamina downward, on a Nuclepore filter (poresize, 0.1 micron). (2) Chondroitin sulfate (CS), the predominant proteoglycan (greater than 85%), seems to play no significant role in collagen fibrillogenesis in vitro. Stromas laid down in its absence were indistinguishable from controls as assayed by fibril diameter, organization, and spacing and the amount of collagen synthesized. For these experiments, epithelia were cultured in the presence of hyaluronidase, which degrades CS, and p-nitrophenyl beta-D-xyloside, w...Continue Reading

References

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Citations

Nov 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C X CaiT F Linsenmayer
Feb 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·F RuggieroR Garrone
Oct 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·M P MarkJ V Ruch

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