Does single-layer centrifugation with Bovicoll improve sperm quality of frozen-thawed semen in Fleckvieh bulls?

Reproduction in Domestic Animals = Zuchthygiene
Joanna SzlendakRicardo Faundez

Abstract

The aim of the present study was to evaluate the effect of sperm selection by single-layer centrifugation (SLC) performed before freezing on sperm quality after thawing of Fleckvieh bull semen. Ejaculates from 22 bulls were collected by artificial vagina and divided into two aliquots. One aliquot (control sample) was diluted with Steridyl® and frozen over nitrogen vapour in a Digitcool freezer (IMV Technologies). Sperm from the second aliquot (SLC sample) was selected using the SLC technique with Bovicoll colloid and then frozen over nitrogen vapour in a Digitcool freezer. After thawing, both samples (control and SLC) were evaluated by computer-aided sperm analysis (CASA; SCA 6.4 System; Microptic S.L) for sperm motility parameters. Integrity of the plasma membrane (viability), high mitochondrial membrane potential (HMMP) and acrosome integrity were assessed using a Guava® easyCyte flow cytometer (IMV Technologies). Morphological examination of spermatozoa was performed by Differential Interference Contrast microscopy (Leica DMi8). Morphological examination of live, immobilized spermatozoa was analysed under high magnification (≥6,600×). After thawing, the mean sperm viability of the control sample was 51.57%, compared to 40.37...Continue Reading

References

Feb 17, 2009·Animal Reproduction Science·A JohannissonH Rodriguez-Martinez
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Jan 25, 2014·Reproduction in Domestic Animals = Zuchthygiene·J M MorrellM Andersson
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Sep 25, 2014·Reproduction in Domestic Animals = Zuchthygiene·Y YulnawatiJ M Morrell
May 10, 2016·Journal of Animal Science and Biotechnology·Alessia GloriaAlberto Contri
Nov 3, 2016·Zygote : the Biology of Gametes and Early Embryos·Maria Celina AbrahamJane M Morrell

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