Does size matter? Study of performance of pseudo-ELISAs based on molecularly imprinted polymer nanoparticles prepared for analytes of different sizes

The Analyst
C CáceresSergey A Piletsky

Abstract

The aim of this work is to evaluate whether the size of the analyte used as template for the synthesis of molecularly imprinted polymer nanoparticles (nanoMIPs) can affect their performance in pseudo-enzyme linked immunosorbent assays (pseudo-ELISAs). Successful demonstration of a nanoMIPs-based pseudo-ELISA for vancomycin (1449.3 g mol(-1)) was demonstrated earlier. In the present investigation, the following analytes were selected: horseradish peroxidase (HRP, 44 kDa), cytochrome C (Cyt C, 12 kDa) biotin (244.31 g mol(-1)) and melamine (126.12 g mol(-1)). NanoMIPs with a similar composition for all analytes were synthesised by persulfate-initiated polymerisation in water. In addition, core-shell nanoMIPs coated with polyethylene glycol (PEG) and imprinted for melamine were produced in organics and tested. The polymerisation of the nanoparticles was done using a solid-phase approach with the correspondent template immobilised on glass beads. The performance of the nanoMIPs used as replacement for antibodies in direct pseudo-ELISA (for the enzymes) and competitive pseudo-ELISA for the smaller analytes was investigated. For the competitive mode we rely on competition for the binding to the nanoparticles between free analyte and ...Continue Reading

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Mar 19, 2013·Nanoscale·Ewa MoczkoSergey Piletsky
Jul 17, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Ewa MoczkoSergey Piletsky

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Citations

May 16, 2019·Advanced Materials·Huiqi Zhang
Sep 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Run-Tian MaYan-Ping Shi
Nov 10, 2020·Critical Reviews in Analytical Chemistry·Matheus Siqueira SilvaEduardo Costa Figueiredo
Dec 8, 2021·Analytical Chemistry·Yadiris GarcíaEduardo D Pereira

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