Does the Pre-Ovulatory Pig Oviduct Rule Sperm Capacitation In Vivo Mediating Transcriptomics of Catsper Channels?

International Journal of Molecular Sciences
Cristina A MartinezH Rodriguez-Martinez

Abstract

Spermatozoa need to conduct a series of biochemical changes termed capacitation in order to fertilize. In vivo, capacitation is sequentially achieved during sperm transport and interaction with the female genital tract, by mechanisms yet undisclosed in detail. However, when boar spermatozoa are stored in the tubal reservoir pre-ovulation, most appear to be in a non-capacitated state. This study aimed at deciphering the transcriptomics of capacitation-related genes in the pig pre-ovulatory oviduct, following the entry of semen or of sperm-free seminal plasma (SP). Ex-vivo samples of the utero-tubal junction (UTJ) and isthmus were examined with a microarray chip (GeneChip® Porcine Gene 1.0 ST Array, Thermo Fisher Scientific) followed by bioinformatics for enriched analysis of functional categories (GO terms) and restrictive statistics. The results confirmed that entry of semen or of relative amounts of sperm-free SP modifies gene expression of these segments, pre-ovulation. It further shows that enriched genes are differentially associated with pathways relating to sperm motility, acrosome reaction, single fertilization, and the regulation of signal transduction GO terms. In particular, the pre-ovulation oviduct stimulates the Ca...Continue Reading

References

Dec 1, 1983·Journal of Dairy Science·H W Hawk
Aug 29, 1998·The Journal of Biological Chemistry·A FelicielloM E Gottesman
Jun 4, 1999·Trends in Cell Biology·M Colledge, J D Scott
Jul 5, 2001·Seminars in Immunology·S A Robertson, D J Sharkey
Sep 26, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·H C Ho, S S Suarez
Dec 4, 2001·Journal of Reproductive Immunology·P E ViscontiA B Diekman
Jan 5, 2002·Theriogenology·H Niemann, D Rath
Apr 20, 2002·Zygote : the Biology of Gametes and Early Embryos·R H F Hunter, H Rodriguez-Martinez
Jun 26, 2003·European Journal of Human Genetics : EJHG·Nili AvidanJacques S Beckmann
Aug 23, 1952·Nature·C R AUSTIN
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Anne E CarlsonDonner F Babcock
Dec 25, 2003·Molecular Reproduction and Development·R H F Hunter, H Rodriguez-Martinez
Mar 24, 2004·Animal Reproduction Science·P TienthaiH Rodriguez-Martinez
Jan 1, 2005·Theriogenology·Heriberto Rodríguez-MartínezJuan J Calvete
Jan 22, 2005·The EMBO Journal·Kazutoyo TeradaMasataka Mori
Jan 18, 2006·Molecular and Cellular Endocrinology·Kenneth P RobertsDavid W Hamilton
Jul 11, 2006·Zygote : the Biology of Gametes and Early Embryos·Ann-Sofi BergqvistHeriberto Rodríguez-Martínez
Nov 25, 2006·Biology of Reproduction·Natsuko Kawano, Manabu Yoshida
Jan 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Huayu QiDavid E Clapham
Apr 25, 2007·Theriogenology·H Rodriguez-Martinez
May 5, 2007·The Journal of Biological Chemistry·Jin LiuDejian Ren
Nov 17, 2007·Journal of Proteome Research·A Stephen GeorgiouAlireza Fazeli
Apr 19, 2008·Cell Motility and the Cytoskeleton·Amy E Hanlon NewellDaniel W Carr
Jun 24, 2008·Developmental Biology·Vanina G Da RosPatricia S Cuasnicu
Jul 24, 2008·The International Journal of Developmental Biology·Betsy NavarroDavid E Clapham
Jul 24, 2008·The International Journal of Developmental Biology·Débora J CohenPatricia S Cuasnicu
Aug 5, 2008·Reproduction in Domestic Animals = Zuchthygiene·E A LandersD J Miller
Aug 30, 2008·Biology of Reproduction·Natsuko KawanoManabu Yoshida
Sep 4, 2010·Veterinary Medicine International·J M Morrell, H Rodriguez-Martinez
Sep 21, 2010·Systems Biology in Reproductive Medicine·Janice L Bailey
Mar 18, 2011·Nature·Polina V LishkoYuriy Kirichok
Mar 29, 2011·Journal of Andrology·Débora J CohenPatricia S Cuasnicú

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Citations

Jul 31, 2020·International Journal of Molecular Sciences·Jaume GardelaManuel Alvarez-Rodriguez
Aug 6, 2020·International Journal of Molecular Sciences·Manuel Alvarez-RodriguezHeriberto Rodriguez-Martinez
Aug 3, 2021·Frontiers in Cell and Developmental Biology·Mari-Carmen Fuentes-AlberoFrancisco Alberto García-Vázquez

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Methods Mentioned

BETA
chip

Software Mentioned

Transcriptome Analysis Console ( TAC
Cytoscape
Database for Annotation , Visualization and Integrated Discove...
limma
CluePedia
Affymetrix
Biorender
SmarArt

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