PMID: 6168468Jul 1, 1981Paper

Dolichol pathway in lymphocytes from rat spleen. Influence of the glucosylation on the cleavage of dolichyl diphosphate oligosaccharides into phosphooligosaccharides

European Journal of Biochemistry
B HoflackA Verbert

Abstract

Incubation of rat-spleen lymphocytes with UDP-glucose together with GDP-mannose and UDP-N-acetylglucosamine leads to the formation of glucosylated lipid intermediates characterized as dolichyl phosphate glucose and dolichyl diphosphate oligosaccharides. This latter can be either transferred onto endogenous protein acceptors or cleaved into phosphooligosaccharides. The striking fact is that phosphooligosaccharide populations contain far less glucosylated products than the dolichyl diphosphate oligosaccharide ones from which they are derived. Two hypotheses have been investigated: either a rapid action of glucosidases on the liberated phosphooligosaccharides or a preferential splitting of the non-glucosylated population of dolichyl diphosphate oligosaccharides. Addition of p-nitrophenyl-alpha-D-glucoside inhibits glucosidase activities and allows the production of a major population of dolichyl diphosphate oligosaccharides containing three glucose residues. Using these conditions, it is shown that the amount of phosphooligosaccharides generated from the splitting of dolichyl diphosphate oligosaccharides is greatly decreased and that the major part of these remaining phosphooligosaccharides do not contain glucose. These results sh...Continue Reading

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S J TurcoP W Robbins
Jan 1, 1978·Methods in Enzymology·N H Behrens, E Tábora
Mar 15, 1976·FEBS Letters·R CacanJ Montreuil
May 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·N H Behrens, L F Leloir
Nov 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·N H BehrensL F Leloir
May 1, 1980·European Journal of Biochemistry·R CacanA Verbert
Apr 1, 1980·European Journal of Biochemistry·R J StaneloniL F Leloir

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Citations

May 15, 1984·European Journal of Biochemistry·D Brada, U C Dubach
Jul 15, 1995·European Journal of Biochemistry·B Kalz-FüllerE Bause
Aug 15, 1989·European Journal of Biochemistry·E BauseB Orthen
Jan 26, 2008·Glycobiology·Isabelle Chantret, Stuart E H Moore
Jan 1, 1988·American Journal of Medical Genetics. Supplement·R K PullarkatV K Patel

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