PMID: 2500966Apr 4, 1989Paper

Domain- and sequence-specific phosphorylation of vimentin induces disassembly of the filament structure

Biochemistry
S AndoMasaki Inagaki

Abstract

We reported that stoichiometric phosphorylation by either cAMP-dependent protein kinase or protein kinase C induces disassembly of vimentin filaments [Inagaki, M., Nishi, Y., Nishizawa, K., Matsuyama, M., & Sato, C. (1987) Nature 328, 649-652; Inagaki, M., Gonda, Y., Matsuyama, M., Nishizawa, K., Nishi, Y., & Sato, C. (1988) J. Biol. Chem. 263, 5970-5978]. In the present work, we attempted to identify the sites of vimentin phosphorylated by each protein kinase. Sequential analysis of the purified phosphopeptides, together with the known primary sequence, revealed that Ser-8, Ser-9, Ser-20, Ser-25, Ser-33, and Ser-41 were specifically phosphorylated by protein kinase C, whereas Ser-46 was phosphorylated preferentially by cAMP-dependent protein kinase. Both kinases reacted with Ser-6, Ser-24, Ser-38, Ser-50, and Ser-65. Specific phosphorylation sites for protein kinase C are mostly located close to the amino-terminal side of arginine while those for cAMP-dependent protein kinase are located close to the carboxyl-terminal side of arginine. The phosphorylation sites exclusively occur in the amino-terminal non-alpha-helical head domain, particularly at the beta-turn region. These results provide clues to the molecular mechanisms of ...Continue Reading

References

Apr 30, 1976·Biochimica Et Biophysica Acta·H Mohri
Jan 1, 1978·Annual Review of Biochemistry·P Y Chou, G D Fasman
Jan 1, 1974·CRC Critical Reviews in Biochemistry·T D Pollard, R R Weihing
Jan 1, 1973·Annual Review of Biochemistry·J B Olmsted, G G Borisy
Sep 1, 1968·The Journal of Cell Biology·H IshikawaH Holtzer
Nov 1, 1983·Cell·W QuaxH Bloemendal
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T Hunter, B M Sefton
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·W A SpruillA L Kierszenbaum
Nov 1, 1982·Experimental Cell Research·S H Blose, A Bushnell
Oct 8, 1955·Nature·C H LIJ S DIXON

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Citations

Jan 1, 1996·Cell Motility and the Cytoskeleton·B S Eckert, P L Yeagle
Jan 1, 1992·Cell Motility and the Cytoskeleton·R A Nixon, T B Shea
Feb 1, 1991·Journal of Cellular Physiology·E M RosenI D Goldberg
Jun 4, 2011·Cellular and Molecular Life Sciences : CMLS·Arun Satelli, Shulin Li
Dec 1, 1993·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·J Ciesielski-TreskaD Aunis
Jan 5, 2002·Journal of Chromatography. a·A KugimiyaT Takeuchi
Mar 29, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Masaaki TakemuraShigeyoshi Itohara
Sep 22, 1999·European Journal of Biochemistry·J TözsérT D Copeland
Nov 1, 1990·The Journal of Cell Biology·J M RaatsH Bloemendal
Nov 1, 1992·The Journal of Cell Biology·J A DentM W Klymkowsky
Mar 1, 1993·The Journal of Cell Biology·H HennekesE A Nigg
Jul 1, 1993·The Journal of Cell Biology·M B McCormickE Fuchs
Sep 1, 1993·The Journal of Cell Biology·M K LeeD W Cleveland
Nov 1, 1995·The Journal of Cell Biology·M OgawaraH Sugiura
Feb 25, 2011·Molecular Biology of the Cell·Brian T HelfandRobert D Goldman
Aug 1, 2012·Glycobiology·Kenta KomuraToshihiro Akaike
Nov 21, 1992·Annals of the New York Academy of Sciences·G Bellomo, F Mirabelli
Feb 17, 2009·Biochemistry. Biokhimii︠a︡·A A Minin, M V Moldaver
Feb 20, 2010·PloS One·Ali BouamraniJean-Paul Issartel
May 1, 1992·Journal of Neurochemistry·A S BenderM D Norenberg
Jan 1, 1990·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·M Kasper, P Stosiek

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