Domain architecture and structure of the bacterial cell division protein DivIB.

Proceedings of the National Academy of Sciences of the United States of America
Scott A Robson, Glenn F King

Abstract

Bacterial cytokinesis requires the coordinated assembly of a complex of proteins, collectively known as the divisome, at the incipient division site. DivIB/FtsQ is a conserved component of the divisome in bacteria with cell walls, suggesting that it plays a role in synthesis and/or remodeling of septal peptidoglycan. We demonstrate that the extracytoplasmic region of DivIB comprises three discrete domains that we designate alpha, beta, and gamma from the N to C terminus. The alpha-domain is proximal to the cytoplasmic membrane and coincident with the polypeptide transport-associated domain that was proposed previously to function as a molecular chaperone. The beta-domain has a unique 3D fold, with no eukaryotic counterpart, and we show that it interconverts between two discrete conformations via cis-trans isomerization of a Tyr-Pro peptide bond. We propose that this isomerization might modulate protein-protein interactions of the flanking alpha- and gamma-domains. The C-terminal gamma-domain is unstructured in the absence of other divisomal proteins, but we show that it is critical for DivIB function.

References

Sep 5, 1993·Journal of Molecular Biology·L Holm, C Sander
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Feb 1, 1996·Journal of Molecular Graphics·R KoradiK Wüthrich
May 1, 1997·Journal of Bacteriology·M A de PedroH Schwarz
Jan 1, 1997·Annual Review of Biochemistry·J Lutkenhaus, S G Addinall
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·C Goffin, J M Ghuysen
Jan 12, 1999·Journal of Bacteriology·J C ChenJ Beckwith
Nov 4, 2000·Nature Structural Biology·G F KingL I Rothfield
Dec 26, 2001·Biochimica Et Biophysica Acta·P Jarvis, J Soll
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jesse StrickerHarold P Erickson
Aug 16, 2002·Biochimica Et Biophysica Acta·Paul Jarvis, Jürgen Soll
Oct 9, 2002·Current Protein & Peptide Science·R Hertle
Nov 30, 2002·Current Opinion in Microbiology·Nienke Buddelmeijer, Jon Beckwith
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Jeffery ErringtonDirk-Jan Scheffers
Oct 7, 2003·Annual Review of Microbiology·Laura Romberg, Petra Anne Levin
Oct 16, 2003·Trends in Biochemical Sciences·Luis Sánchez-PulidoAlfonso Valencia
Apr 23, 2004·Molecular Microbiology·Ji-Chun YangJan Löwe
Aug 20, 2004·Methods in Molecular Biology·Peter Güntert
Oct 20, 2004·Molecular Microbiology·David S Weiss
Mar 19, 2005·Molecular Microbiology·Shin-Ya MiyagishimaKatherine W Osteryoung
Jul 12, 2005·Current Biology : CB·Nathan W Goehring, Jon Beckwith
Aug 23, 2005·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Petra Jilma-StohlawetzBernd Jilma
Oct 18, 2005·Nature Reviews. Molecular Cell Biology·William Margolin

❮ Previous
Next ❯

Citations

Mar 8, 2008·Nature Reviews. Drug Discovery·Rowena L Lock, Elizabeth J Harry
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jennifer J SmithPaul F Alewood
Jul 29, 2009·The Journal of Biological Chemistry·Soizic MassonAndré Zapun
Apr 29, 2008·Journal of Bacteriology·Audrey Le GouëllecAndré Zapun
Aug 19, 2007·Journal of Bacteriology·Dirk-Jan ScheffersJoen Luirink
May 29, 2010·Microbiology and Molecular Biology Reviews : MMBR·Andrew RobinsonNicholas E Dixon
Jun 16, 2011·BMC Structural Biology·Felipe VillaneloOctavio Monasterio
May 13, 2010·New Biotechnology·L GrengaL Paolozzi
Mar 4, 2008·Molecular Microbiology·Fusinita van den EntJan Löwe
Jun 5, 2013·Environmental Microbiology·Seungdae OhKonstantinos T Konstantinidis
Feb 23, 2008·FEMS Microbiology Reviews·Tanneke den BlaauwenJuan A Ayala
Feb 13, 2008·FEMS Microbiology Reviews·André ZapunMariana G Pinho
Oct 20, 2012·Australasian Psychiatry : Bulletin of Royal Australian and New Zealand College of Psychiatrists·Saxby Pridmore, Garry Walter
Jan 10, 2018·Journal of Bacteriology·Katherine J WuCara C Boutte
Jun 16, 2018·The Journal of Biological Chemistry·Preeti JainVinay Kumar Nandicoori
Sep 10, 2019·Protein Science : a Publication of the Protein Society·Simon Booth, Richard J Lewis
Nov 29, 2008·Biotechnology and Bioengineering·Rajesh K SrivastavaPramod P Wangikar
Aug 16, 2018·Cellular and Molecular Life Sciences : CMLS·Bruno MadioEivind A B Undheim

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.