Domain behavior during the folding of a thermostable phosphoglycerate kinase

Biochemistry
M J ParkerA R Clarke

Abstract

Bacillus stearothermophilus phosphoglycerate kinase (bsPGK) is a monomeric enzyme of 394 residues comprising two globular domains (N and C), covalently linked by an interdomain alpha-helix (residues 170-185). The molecule folds to the native state in three stages. In the first, each domain rapidly and independently collapses to form an intermediate in which the N-domain is stabilized by 5.1 kcal mol-1 and the C-domain by 3.3 kcal mol-1 over their respective unfolded conformations. The N-domain then converts to a folded state at a rate of 1.2 s-1 (delta GI-F = 3.8 kcal mol-1), followed by the C-domain at 0.032 s-1 (delta GI-F = 12.1 kcal mol-1). It is this last step that limits the rate of acquisition of enzyme activity. In the dynamics of unfolding in water, the N-domain converts to the intermediate state at a rate of 8 x 10(-4) s-1, some 10(7) times faster than the C-domain. Consequently, the most populated intermediate in the folding reaction has a native-like N-domain, while that in the unfolding direction has a native-like C-domain. In a conventional sense, therefore, the folding/unfolding kinetics of bsPGK can be described as random order. Consistent with these observations, cutting the molecule in the interdomain helix pr...Continue Reading

References

Oct 1, 1989·European Journal of Biochemistry·W J FairbrotherR J Williams
Jan 10, 1973·Journal of Molecular Biology·A IkaiC Tanford
Oct 25, 1980·Journal of Molecular Biology·F SangerB A Roe
Jul 28, 1995·Journal of Molecular Biology·N A RansonA R Clarke
Jan 1, 1996·Folding & Design·R L Baldwin

❮ Previous
Next ❯

Citations

Dec 11, 1999·Cell Biochemistry and Biophysics·S KumarR Nussinov
Oct 1, 2003·Journal of Molecular Biology·Szabolcs OsváthMartin Gruebele
Jul 16, 2003·Journal of Molecular Biology·Michelle A C ReedJonathan P Waltho
Mar 31, 1999·Progress in Biophysics and Molecular Biology·R Jaenicke
Apr 21, 1999·Biochimica Et Biophysica Acta·G S JacksonJ Collinge
Aug 1, 1997·Current Opinion in Biotechnology·A R Clarke, J P Waltho
Oct 23, 1997·Nature Structural Biology·L L HosszuJ P Waltho
Mar 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Tanya HartAnthony R Clarke
Nov 16, 2010·Protein Engineering, Design & Selection : PEDS·Hauke LilieThomas Kriegel
Apr 7, 2000·Protein Science : a Publication of the Protein Society·O V GalzitskayaH Nakamura
May 14, 2009·HFSP Journal·Sarah BateyJane Clarke
Jan 10, 2002·Journal of Molecular Biology·Louise A Wallace, C Robert Matthews
Jan 3, 2006·Biophysical Journal·Sarah BateyJane Clarke
Mar 31, 2004·Journal of Molecular Biology·Ignacio E SánchezRudi Glockshuber
Jan 25, 2006·Journal of Molecular Biology·Michelle A C ReedJonathan P Waltho
Jul 30, 2003·Biophysical Journal·Szabolcs Osváth, Martin Gruebele
Mar 5, 2015·Journal of Biomolecular NMR·Peter KiralyGareth A Morris
May 11, 2005·Protein Science : a Publication of the Protein Society·Szabolcs OsváthJudit Fidy
May 31, 2019·Extremophiles : Life Under Extreme Conditions·David MandelmanNushin Aghajari
Aug 29, 1998·The Journal of Biological Chemistry·J R MattinglyM Martinez-Carrion
Feb 9, 2019·Scientific Reports·Balachandran ManavalanJooyoung Lee
Nov 28, 2008·Biochemistry·Lilia MilanesiJonathan P Waltho

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.