PMID: 9161041Apr 1, 1997Paper

Domain exchanges between KNAT3 and KNAT1 suggest specificity of the kn1-like homeodomains requires sequences outside of the third helix and N-terminal arm of the homeodomain

The Plant Journal : for Cell and Molecular Biology
K A Serikawa, P C Zambryski

Abstract

Domain exchange constructs that traded regions surrounding the homeodomain were constructed for two kn1-like genes, KNAT1 and KNAT3, and introduced into Arabidopsis thaliana under the control of the 35S CaMV promoter. The kn1-like homeodomain proteins all have the homeodomain located near the C-terminus of the protein, and also share a second conserved domain (the ELK domain) immediately N-terminal to the homeodomain. Progeny were scored for the appearance of the KNAT1 overexpression phenotype. A construct containing the KNAT3 N-terminus and the KNAT1 ELK- and homeodomain resulted in a KNAT1 overexpression phenotype, indicating that specificity mainly resides within the ELK- and homeodomain region. Further exchanges demonstrated that specificity probably does not arise from a single region within the ELK and/or homeodomain but rather requires sequences both N-terminal and C-terminal to residue 23 of the homeodomain. Further, in contrast to some animal homeodomains, KNAT1 does not utilize the residues of the N-terminal arm of the homeodomain for specificity.

Citations

Jan 1, 2002·The Arabidopsis Book·Hirokazu Tsukaya
Jun 10, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R W Williams
May 26, 2006·Plant Molecular Biology·Simon Scofield, James A H Murray
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Neelima Sinha
Oct 10, 1998·Biochimica Et Biophysica Acta·R L ChanD H Gonzalez

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