Domain-specific early and late function of Dpatj in Drosophila photoreceptor cells

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Sang-Chul Nam, Kwang-Wook Choi

Abstract

The formation and maintenance of cell polarity is essential for epithelial morphogenesis. Dpatj (Drosophila homolog of mammalian Patj) is a multi-PDZ domain protein that localizes to the apical cell membrane and forms a protein complex with cell polarity proteins, Crumbs (Crb) and Stardust (Sdt). Whereas Crb and Sdt are known to be required for the organization of adherens junctions (AJs) and rhabdomeres in differentiating photoreceptors, the in vivo function of Dpatj as a member of the Crb complex in developing eye has been unclear due to the lack of loss-of-function mutations specifically affecting the dpatj gene. Our genetic analysis of hypomorph, null, and RNA interference reveals distinct dual functions of Dpatj in developing and mature photoreceptors. The C-terminal region (PDZ domains 2-4) of Dpatj is not essential for development of the animal but is required to prevent late-onset photoreceptor degeneration. In contrast, the N-terminal region of Dpatj is essential for animal viability and photoreceptor morphogenesis during development. The localization and maintenance of Crb and Sdt in the apical photoreceptor membrane are strongly affected by reduced levels of Dpatj. Dpatj is necessary for proper positioning of AJs and...Continue Reading

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Citations

Oct 17, 2009·Cell and Tissue Research·Ling-Yu LiuSeng-Sheen Fan
Sep 24, 2010·Molecular Biology of the Cell·Natalia A BulgakovaElisabeth Knust
Mar 4, 2009·Molecular and Cellular Biology·Makoto AdachiShoichiro Tsukita
Jan 30, 2010·Development·Emily C N Richardson, Franck Pichaud
Oct 25, 2011·PloS One·Uyen Ngoc MuiSang-Chul Nam
Nov 7, 2012·The Journal of Cell Biology·Arnab SenMichael P Krahn
Apr 15, 2008·Experimental Eye Research·Ilse GosensRonald Roepman
Oct 16, 2007·Current Opinion in Neurobiology·Elisabeth Knust
Dec 1, 2006·Trends in Cell Biology·Ulrich Tepass, Kathryn P Harris
Aug 13, 2009·Genesis : the Journal of Genetics and Development·Tony W ChenSang-Chul Nam
May 28, 2010·Genesis : the Journal of Genetics and Development·Bibhash MukhopadhyayKwang-Wook Choi
Nov 17, 2007·Biochimica Et Biophysica Acta·Emeline AssématDominique Massey-Harroche
Feb 11, 2015·The Biochemical Journal·Dan JinAlan Hall
Jan 12, 2010·Mechanisms of Development·Christian WenzlJörg Grosshans
Nov 20, 2012·Current Biology : CB·Jennifer J Hwa, Thomas R Clandinin
Jun 1, 2010·Cell·Daniel St Johnston, Julie Ahringer
Sep 1, 2016·Genesis : the Journal of Genetics and Development·Sang-Chul Nam
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Mary Anna CarboneTrudy F C Mackay
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Nov 9, 2012·Development·Clothilde Pénalva, Vincent Mirouse
Sep 3, 2021·Neuropsychiatric Disease and Treatment·Wen-Jie WangZixiao Li

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