PMID: 9171419Jun 1, 1997Paper

Domains of Escherichia coli acyl carrier protein important for membrane-derived-oligosaccharide biosynthesis

Journal of Bacteriology
L TangE P Kennedy

Abstract

Acyl carrier protein participates in a number of biosynthetic pathways in Escherichia coli: fatty acid biosynthesis, phospholipid biosynthesis, lipopolysaccharide biosynthesis, activation of prohemolysin, and membrane-derived oligosaccharide biosynthesis. The first four pathways require the protein's prosthetic group, phosphopantetheine, to assemble an acyl chain or to transfer an acyl group from the thioester linkage to a specific substrate. By contrast, the phosphopantetheine prosthetic group is not required for membrane-derived oligosaccharide biosynthesis, and the function of acyl carrier protein in this biosynthetic scheme is currently unknown. We have combined biochemical and molecular biological approaches to investigate domains of acyl carrier protein that are important for membrane-derived oligosaccharide biosynthesis. Proteolytic removal of the first 6 amino acids from acyl carrier protein or chemical synthesis of a partial peptide encompassing residues 26 to 50 resulted in losses of secondary and tertiary structure and consequent loss of activity in the membrane glucosyltransferase reaction of membrane-derived oligosaccharide biosynthesis. These peptide fragments, however, inhibited the action of intact acyl carrier ...Continue Reading

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Citations

Oct 3, 2006·The Journal of Biological Chemistry·Salma RafiPeter J Tonge
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrik JohanssonMartin Grininger
Dec 7, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·David M Byers, Huansheng Gong
Nov 15, 2006·The Journal of Biological Chemistry·Padmesh VenkitasubramanianJohn P N Rosazza
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·P StanleyC Hughes
Feb 21, 2003·Biochemical and Biophysical Research Communications·Huansheng Gong, David M Byers
Feb 4, 2006·Biochemical and Biophysical Research Communications·Yangmee KimZiad Eletr
Mar 4, 2006·Science·Simon JenniNenad Ban

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