Dominance of Mating Type A1 and Indication of Epigenetic Effects During Early Stages of Mating in Phytophthora infestans

Frontiers in Microbiology
Georgios TzelepisChristina Dixelius

Abstract

The potato late blight pathogen Phytophthora infestans has both an asexual and a sexual mode of reproduction. In Scandinavia, the pathogen is reproducing sexually on a regular basis, whereas clonal lineages dominate in other geographical regions. This study aimed at elucidating events or key genes underlying this difference in sexual behavior. First, the transcriptomes of eight strains, known as either clonal or sexual, were compared during early stages of mating. Principal component analysis (PCA) divided the samples in two clusters A and B and a clear grouping of the mating samples together with the A1 mating type parents was observed. Induction of genes encoding DNA adenine N6-methylation (6mA) methyl-transferases clearly showed a bias toward the cluster A. In contrast, the Avrblb2 effector gene family was highly induced in most of the mating samples and was associated with cluster B in the PCA, similarly to genes coding for acetyl-transferases, which play an important role in RXLR modification prior to secretion. Avrblb2 knock-down strains displayed a reduction in virulence and oospore formation, suggesting a role during the mating process. In conclusion, a number of gene candidates important for the reproductive processes ...Continue Reading

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Citations

Apr 21, 2021·Environmental Microbiology Reports·Fernando Uriel Rojas-Rojas, Julio C Vega-Arreguín

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Datasets Mentioned

BETA
PRJNA17665
GSE94137

Methods Mentioned

BETA
RNA-seq
RNAseq
PCA
acetylation
transgenic

Software Mentioned

Picard
R package matlib
HTSeq count
package
GATK
package Genefilter
Genefinder
SPSS
Cufflinks
AVR3a

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