PMID: 9174133May 1, 1997Paper

Dominant clones in immortalized T-cell lines from rheumatoid arthritis synovial membranes

Tissue Antigens
A M SaadawiM Thomsen

Abstract

Transformation of human T cells by herpesvirus saimiri allows the production of an unlimited number of T cells which express a functional T-cell receptor. In this study we transformed four T-cell lines derived from rheumatoid arthritis synovial membranes. The transformed T cells were mainly CD4+ and expressed the phenotype of activated T cells. They were grown for more than 1 year in the absence of mitogen or feeder cells, and three of them could be maintained without exogenous IL-2. The presence of viral DNA in the transformed cells was shown by in situ hybridization with a probe from the H-DNA region of the virus. No infectious virus could be recovered from the transformed cells. The relative proportion of the 24 different Vbeta families between the four transformed lines showed variations that increased with time. In the two T-cell lines transformed at an early stage of culture, the Vbeta2 family was maintained at about 10%. The dominant Vbeta2 clones that previously have been characterized in the patient were found in all transformed T-cell lines. We have thus shown the feasibility of obtaining transformed T cells from synovial membranes. They contain the dominant clones that are considered of potential importance for the d...Continue Reading

References

Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G J TodaroB Fleckenstein
Apr 20, 1978·The New England Journal of Medicine·P Stastny
Nov 30, 1979·Biochimica Et Biophysica Acta·B Fleckenstein
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B BiesingerB Fleckenstein
Sep 1, 1992·The Journal of Experimental Medicine·H W MittrückerB Fleischer
Aug 1, 1992·The Journal of Clinical Investigation·W V WilliamsD B Weiner
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J U JungR C Desrosiers
Dec 1, 1991·Journal of Virology·J U Jung, R C Desrosiers
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B P WordsworthJ I Bell
Apr 1, 1989·Journal of Virology·R Grassmann, B Fleckenstein
Jul 1, 1985·Journal of Virology·A T BankierW Bodemer
Jul 1, 1982·Journal of Virology·R C Desrosiers, L A Falk
Oct 22, 1981·The New England Journal of Medicine·B L KotzinH S Kaplan
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·F WeberR Hohlfeld
Feb 1, 1995·Immunology Today·E MeinlB Fleckenstein

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Citations

Feb 16, 2000·Journal of Neuroimmunology·E Meinl, R Hohlfeld
Mar 27, 1999·Oral Microbiology and Immunology·M A SolM Thomsen
Sep 25, 2004·Journal of Cellular Physiology·Alexander Y Tsygankov
Dec 2, 2009·Arthritis and Rheumatism·Esther WilkRoland Jacobs
Jan 16, 2004·The Journal of Biological Chemistry·Elena M SorokinaAlexander Y Tsygankov

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