Dominant fecal microbiota in newly diagnosed untreated inflammatory bowel disease patients

Gastroenterology Research and Practice
Lill Therese ThorkildsenKnut Rudi

Abstract

Our knowledge about the microbiota associated with the onset of IBD is limited. The aim of our study was to investigate the correlation between IBD and the fecal microbiota for early diagnosed untreated patients. The fecal samples used were a part of the Inflammatory Bowel South-Eastern Norway II (IBSEN II) study and were collected from CD patients (n = 30), UC patients (n = 33), unclassified IBD (IBDU) patients (n = 3), and from a control group (n = 34). The bacteria associated with the fecal samples were analyzed using a direct 16S rRNA gene-sequencing approach combined with a multivariate curve resolution (MCR) analysis. In addition, a 16S rRNA gene clone library was prepared for the construction of bacteria-specific gene-targeted single nucleotide primer extension (SNuPE) probes. The MCR analysis resulted in the recovery of five pure components of the dominant bacteria present: Escherichia/Shigella, Faecalibacterium, Bacteroides, and two components of unclassified Clostridiales. Escherichia/Shigella was found to be significantly increased in CD patients compared to control subjects, and Faecalibacterium was found to be significantly reduced in CD patients compared to both UC patients and control subjects. Furthermore, a SNu...Continue Reading

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Citations

Jan 24, 2016·Journal of Crohn's & Colitis·Mireia Valles-ColomerJeroen Raes
Sep 5, 2015·Gut·Julian R MarchesiAilsa Hart
Apr 23, 2016·International Journal of Molecular Sciences·Mario MatijašićDonatella Verbanac
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Oct 31, 2019·United European Gastroenterology Journal·Fatouma SalemDavid Moulin
Nov 20, 2020·The Science of the Total Environment·Huifeng XieChengyong Li
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May 25, 2021·Frontiers in Cellular and Infection Microbiology·Shih-Cheng ChangJang-Jih Lu
Jun 22, 2021·Frontiers in Immunology·Heather M Grifka-WalkDouglas J Kominsky

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Methods Mentioned

BETA
PCR
PCA
electrophoresis
dot blot

Software Mentioned

BioNJ
AIBIMM
GA
CLC Main Workbench
GeneMapper
PhyloMode
Dialister
TNTProbeTool
CLC
Dendroscope

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