PMID: 9658167Jul 11, 1998Paper

Dominant negative alleles of SEC10 reveal distinct domains involved in secretion and morphogenesis in yeast

Molecular Biology of the Cell
D RothPeter Novick

Abstract

The accurate targeting of secretory vesicles to distinct sites on the plasma membrane is necessary to achieve polarized growth and to establish specialized domains at the surface of eukaryotic cells. Members of a protein complex required for exocytosis, the exocyst, have been localized to regions of active secretion in the budding yeast Saccharomyces cerevisiae where they may function to specify sites on the plasma membrane for vesicle docking and fusion. In this study we have addressed the function of one member of the exocyst complex, Sec10p. We have identified two functional domains of Sec10p that act in a dominant-negative manner to inhibit cell growth upon overexpression. Phenotypic and biochemical analysis of the dominant-negative mutants points to a bifunctional role for Sec10p. One domain, consisting of the amino-terminal two-thirds of Sec10p directly interacts with Sec15p, another exocyst component. Overexpression of this domain displaces the full-length Sec10 from the exocyst complex, resulting in a block in exocytosis and an accumulation of secretory vesicles. The carboxy-terminal domain of Sec10p does not interact with other members of the exocyst complex and expression of this domain does not cause a secretory defe...Continue Reading

References

May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J E GerstM Wigler
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Aug 1, 1981·Cell·P NovickR Schekman
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·A E TingR H Scheller
Mar 1, 1995·The Journal of Cell Biology·B GovindanP Novick
Nov 3, 1994·Nature·J E Rothman
Jan 1, 1994·Annual Review of Biochemistry·M K Bennett, R H Scheller
Dec 15, 1994·Nature·J P LianS Ferro-Novick
Jul 21, 1994·Nature·S Ferro-Novick, R Jahn
Aug 1, 1993·Current Opinion in Cell Biology·M Zerial, H Stenmark
Mar 25, 1993·Nature·T SöllnerJ E Rothman
Apr 12, 1996·Science·J E Rothman, F T Wieland
Feb 9, 1996·Cell·D G Drubin, W J Nelson
Dec 1, 1996·Neuron·S C HsuR H Scheller

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Citations

May 10, 2000·Trends in Cell Biology·W GuoP Novick
Jul 20, 2002·Seminars in Cell & Developmental Biology·Hao XuWilliam S Trimble
Feb 21, 2002·Molecular Biology of the Cell·Hongyan WangMohan K Balasubramanian
Apr 10, 1999·Molecular Biology of the Cell·S L Reck-PetersonM S Mooseker
May 22, 2008·The Plant Cell·Michal HálaViktor Zárský
Feb 14, 2009·PloS One·Nicole J CroteauMary Munson
Apr 15, 2014·PloS One·Nemanja VukašinovićLukáš Synek
Dec 10, 2003·The Journal of Cell Biology·Magali PrigentPhilippe Chavrier
Aug 12, 1998·The Journal of Cell Biology·F P Finger, P Novick
Nov 20, 2015·Molecular Biology of the Cell·Cecília SeixasDuarte C Barral
Dec 15, 2015·Nature Structural & Molecular Biology·Margaret R HeiderMary Munson
May 20, 2009·Fungal Genetics and Biology : FG & B·Aki Yoneda, Tamara L Doering
Aug 17, 1999·Current Opinion in Cell Biology·M G Waters, S R Pfeffer
Mar 24, 2004·International Review of Cytology·Shu-Chan HsuWei Guo
Jan 18, 2017·Scientific Reports·Jianxing ChenShuya Fukai
Apr 3, 2001·Nature Cell Biology·W GuoP Novick
Mar 22, 2012·The Journal of Biological Chemistry·Shanshan FengWei Guo
Oct 10, 2014·American Journal of Physiology. Renal Physiology·Ben FogelgrenJoshua H Lipschutz
Aug 7, 1999·The Journal of Biological Chemistry·W GuoP Novick
Sep 10, 2004·The Biochemical Journal·Crestina L BeitesWilliam S Trimble
Jul 18, 2020·Frontiers in Plant Science·Tamara PečenkováFatima Cvrčková

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