Dominant-negative CK2alpha induces potent effects on circadian rhythmicity.

PLoS Genetics
Elaine M SmithR Allada

Abstract

Circadian clocks organize the precise timing of cellular and behavioral events. In Drosophila, circadian clocks consist of negative feedback loops in which the clock component PERIOD (PER) represses its own transcription. PER phosphorylation is a critical step in timing the onset and termination of this feedback. The protein kinase CK2 has been linked to circadian timing, but the importance of this contribution is unclear; it is not certain where and when CK2 acts to regulate circadian rhythms. To determine its temporal and spatial functions, a dominant negative mutant of the catalytic alpha subunit, CK2alpha(Tik), was targeted to circadian neurons. Behaviorally, CK2alpha(Tik) induces severe period lengthening (approximately 33 h), greater than nearly all known circadian mutant alleles, and abolishes detectable free-running behavioral rhythmicity at high levels of expression. CK2alpha(Tik), when targeted to a subset of pacemaker neurons, generates period splitting, resulting in flies exhibiting both long and near 24-h periods. These behavioral effects are evident even when CK2alpha(Tik) expression is induced only during adulthood, implicating an acute role for CK2alpha function in circadian rhythms. CK2alpha(Tik) expression res...Continue Reading

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Citations

Jul 3, 2008·Genes & Development·Justin Blau
Sep 26, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Rose-Anne MeissnerRavi Allada
Jan 16, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Bikem AktenF Rob Jackson
Mar 20, 2009·PLoS Biology·Michael Rosbash
May 1, 2009·PLoS Biology·Johannes LandskronRalf Stanewsky
Feb 14, 2012·Genes & Development·Eun Young KimJin Won Cho
Dec 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hyeongmin LeeChoogon Lee
Nov 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Philip B KiddEric D Siggia
Sep 11, 2009·Trends in Biochemical Sciences·Arun MehraJay C Dunlap
Feb 19, 2009·Journal of Insect Physiology·Joanna KotwicaJadwiga M Giebultowicz
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Mirko Pegoraro, Eran Tauber
Oct 31, 2009·Journal of Enzyme Inhibition and Medicinal Chemistry·Andreas GratzJoachim Jose
Mar 8, 2011·FEBS Letters·Silke Reischl, Achim Kramer
Oct 21, 2018·Behavior Genetics·Susan T HarbisonTrudy F C Mackay
Jan 7, 2014·PloS One·Shenying FangJeffrey E Lee
May 16, 2019·International Journal of Molecular Sciences·Sara HegaziHai-Ying Mary Cheng
Feb 6, 2021·Current Biology : CB·Yunchuan ZhangYi Zhong

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Methods Mentioned

BETA
nuclear translocation
dissection
transgenic
PCR

Software Mentioned

pdfTik
Clocklab
NIH Image J
Image J
Excel

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