Dominant-negative interference with defence signalling by truncation mutations of the tomato Cf-9 disease resistance gene

The Plant Journal : for Cell and Molecular Biology
Claire L BarkerD A Jones

Abstract

The tomato Cf-9 gene confers resistance to races of the leaf mould fungus Cladosporium fulvum that carry the Avr9 avirulence gene. Cf-9 was isolated by transposon tagging using a modified maize Dissociation (Ds) element. This generated an allelic series of Ds-induced mutations of Cf-9, of which two were found to confer novel phenotypes in a screen for mutants affecting wild-type Cf-9 function in trans. Genetic and molecular analysis of these mutants suggested semidominant, Avr9-dependent, negative-interfering mutations involving Ds insertions in a defined subregion of Cf-9. Interference was associated with expression of the 5'-end of Cf-9 upstream of the Ds insertions in these mutants, suggesting that truncated Cf-9 proteins were the likely cause of interference. Transgenic tomato lines harbouring Cf-9 constructs with premature stop codons in positions similar to the Ds insertions also showed interference, indicating that the presence of Ds was not required for interference to occur. Interestingly, interference in these transgenic lines was completely dominant and was associated with a pronounced developmental phenotype that was dependent on co-expression of Cf-9, Avr9 and a truncated Cf-9 transgene. However, interference with ...Continue Reading

References

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Feb 6, 2004·The Plant Journal : for Cell and Molecular Biology·Xinli SunQifa Zhang

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Citations

Apr 9, 2008·Molecular Plant-microbe Interactions : MPMI·Ahmed J AfzalDavid A Lightfoot
Jun 20, 2014·The Plant Journal : for Cell and Molecular Biology·Severine HurniBeat Keller

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