Dominant-negative Sox18 function inhibits dermal papilla maturation and differentiation in all murine hair types

Development
Rehan VillaniKiarash Khosrotehrani

Abstract

SOX family proteins SOX2 and SOX18 have been reported as being essential in determining hair follicle type; however, the role they play during development remains unclear. Here, we demonstrate that Sox18 regulates the normal differentiation of the dermal papilla of all hair types. In guard (primary) hair dermal condensate (DC) cells, we identified transient Sox18 in addition to SOX2 expression at E14.5, which allowed fate tracing of primary DC cells until birth. Similarly, expression of Sox18 was detected in the DC cells of secondary hairs at E16.5 and in tertiary hair at E18.5. Dominant-negative Sox18 mutation (opposum) did not prevent DC formation in any hair type. However, it affected dermal papilla differentiation, restricting hair formation especially in secondary and tertiary hairs. This Sox18 mutation also prevented neonatal dermal cells or dermal papilla spheres from inducing hair in regeneration assays. Microarray expression studies identified WNT5A and TNC as potential downstream effectors of SOX18 that are important for epidermal WNT signalling. In conclusion, SOX18 acts as a mesenchymal molecular switch necessary for the formation and function of the dermal papilla in all hair types.

References

Feb 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·E D Kaplan, K A Holbrook
Nov 30, 2000·Molecular and Cellular Biology·D PennisiP Koopman
Feb 14, 2002·The Journal of Investigative Dermatology·Sarah E Millar
May 16, 2003·Genesis : the Journal of Genetics and Development·Kristy JamesPeter Koopman
Sep 2, 2003·Neuron·Victoria GrahamLarysa Pevny
Sep 7, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jeff BiernaskieWolfram Tetzlaff
Nov 23, 2007·Circulation Research·Robert HerpersStefan Schulte-Merker
Dec 21, 2007·Blood·Solei CermenatiMonica Beltrame
Feb 22, 2008·Journal of the American Society of Nephrology : JASN·Andrew P McMahonUNKNOWN GUDMAP project
Apr 2, 2008·Developmental Biology·Hélène PendevilleMarianne L Voz
Oct 22, 2008·Nature·Mathias FrançoisPeter Koopman
Sep 15, 2009·Birth Defects Research. Part C, Embryo Today : Reviews·Olivier Duverger, Maria I Morasso
Dec 14, 2012·The Journal of Investigative Dermatology·Mathieu P RoderoKiarash Khosrotehrani
Aug 21, 2013·Developmental Biology·Marta H LeskoFiona M Watt
Oct 25, 2013·Nature Communications·Monica CoradaElisabetta Dejana
Feb 18, 2014·The Journal of Investigative Dermatology·Samantha S HodgsonKiarash Khosrotehrani
Sep 10, 2014·Matrix Biology : Journal of the International Society for Matrix Biology·Ismaïl HendaouiRuth Chiquet-Ehrismann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.