PMID: 8971627Nov 1, 1996Paper

Dominant selection system for use in Cryptococcus neoformans

Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology
G M CoxJ R Perfect

Abstract

Present transformation systems for Cryptococcus neoformans depend on complementation of auxotrophic mutants. We have developed a dominant selection system for transformation of wild-type strains of cryptococci in which resistance to the antibiotic hygromycin B is used as the selectable marker. A heterologous fusion gene construct was created by attaching the putative promoter sequence and start site from a cryptococcal actin gene to a truncated hygromycin B phosphotransferase gene from E. coli. Biolistic transformation with this construct resulted in cryptococci resistant to hygromycin B, and transformation efficiencies approached approximately 500 transformants per microgram DNA. The construct was found to exist in transformants as both extrachromosomal and integrative forms. The transformants with integrated constructs were stable both in vitro and in vivo, and constructs were recoverable from most transformed cells using a plasmid rescue technique. This is the first dominant selection system for use in C. neoformans, and it should prove useful for molecular studies with this important pathogenic yeast.

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Jul 1, 1995·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·G M CoxJ R Perfect
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Citations

Jan 17, 2007·Applied Microbiology and Biotechnology·Bertrand NeveuRichard R Bélanger
Dec 15, 2000·Trends in Microbiology·T L Doering
Aug 22, 2000·Antimicrobial Agents and Chemotherapy·P SoteropoulosD S Perlin
Jan 5, 2000·Clinical and Diagnostic Laboratory Immunology·J HuaJ K Lodge
Jan 20, 2000·Infection and Immunity·G M CoxJ R Perfect
Apr 18, 2000·Infection and Immunity·A L RosasA Casadevall
Aug 14, 2001·Infection and Immunity·X ZhuP R Williamson
Feb 22, 2003·Infection and Immunity·Mairi C NoverrGary B Huffnagle
Apr 22, 2003·Infection and Immunity·P T MageeDana Davis
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·K B LengelerJ Heitman
Mar 15, 2005·The Journal of Clinical Investigation·John PanepintoPeter R Williamson
Feb 10, 2007·The Journal of Clinical Investigation·Scott R WatermanPeter R Williamson
Feb 9, 2008·The Journal of Clinical Investigation·Guowu HuPeter R Williamson
Jan 1, 2014·Yeast·Deepa SrikantaTamara L Doering
Nov 14, 2002·Annual Review of Genetics·Christina M Hull, Joseph Heitman
Oct 3, 2003·The Journal of Clinical Investigation·Chiara LubertoMaurizio Del Poeta
Dec 3, 2014·Fungal Genetics and Biology : FG & B·Nannan ZhangPeter R Williamson
Aug 2, 2005·FEMS Immunology and Medical Microbiology·John R Perfect
Oct 28, 2008·Fungal Genetics and Biology : FG & B·Jack A RuffLorina G Baker
Aug 25, 2004·Clinics in Laboratory Medicine·John W Baddley, Stephen A Moser
Jul 18, 2018·Current Protocols in Microbiology·Kwang-Woo JungYong-Sun Bahn
Sep 24, 2004·The Journal of Biological Chemistry·Cara L GriffithTamara L Doering
Dec 24, 1997·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·D L Toffaletti, J R Perfect
May 15, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alison J EastmanMichal A Olszewski

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