PMID: 8968689Sep 1, 1996Paper

Dominant TCR alpha-chain clonotypes and interferon-gamma are expressed in the pancreas of patients with recent-onset insulin-dependent diabetes mellitus

Diabetes Research and Clinical Practice
K YamagataY Matsuzawa

Abstract

In order to clarify the nature of T lymphocytes infiltrating the pancreatic islets of patients with insulin-dependent diabetes mellitus (IDDM), we analysed T cell receptor (TCR) gene transcripts expressed in pancreatic biopsy specimens of patients with recent-onset IDDM. We also investigated the expression of cytokines (interferon-gamma: IFN-gamma; tumour necrosis factor-alpha: TNF-alpha; interleukin-4: IL-4; interleukin-6: IL-6) in the same specimens. The TCR V beta repertoire was not restricted either in the pancreas or the peripheral lymphocytes of IDDM patients. In contrast, the TCR V alpha repertoire was restricted in the pancreas, but not in the peripheral blood lymphocytes, of IDDM patients. The sequence analysis of the complementarity-determining region 3 (CDR3) of the TCR alpha revealed the presence of dominant clonality in alpha chains of T cells in the patients. IFN-gamma mRNA was highly expressed in the pancreas of IDDM patients, while IL-4 mRNA was deficient. A lower level of expression of IL-6 mRNA was detected in the IDDM pancreas than in the control tissue. These results indicate that T cells bearing a distinct TCR alpha chain are selectively retained and activated within the pancreas of recent-onset IDDM.

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Citations

Jul 2, 1999·Springer Seminars in Immunopathology·P Moss, J Bell
Jan 19, 2017·Current Opinion in Endocrinology, Diabetes, and Obesity·Sally C Kent, Jenny Aurielle B Babon
Dec 4, 2003·Diabetes/metabolism Research and Reviews·Ruben Varela-Calvino, Mark Peakman
Dec 20, 2017·Immunological Investigations·Gamal AllamAdnan A Alsulaimani
Sep 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R Varela-CalvinoM Peakman
Feb 18, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eva Codina-BusquetaMercè Martí
Dec 20, 2007·Applied Immunohistochemistry & Molecular Morphology : AIMM·Monika JablonowskaArtur Milnerowicz
Jul 17, 1998·Clinical and Diagnostic Laboratory Immunology·S N Liossis, G C Tsokos

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