Donor hearts in the Sydney Heart Bank: reliable control but is it 'normal' heart?

Biophysics Reviews
S B MarstonAndrew Messer

Abstract

Human heart samples from the Sydney Heart Bank have become a de facto standard against which others can be measured. Crucially, the heart bank contains a lot of donor heart material: for most researchers this is the hardest to obtain and yet is necessary since we can only study the pathological human heart in comparison with a control, preferably a normal heart sample. It is not generally realised how important the control is for human heart studies. We review our studies on donor heart samples. We report the results obtained with 17 different donor samples collected from 1994 to 2011 and measured from 2005 to 2015 by our standard methodology for in vitro motility and troponin I phosphorylation measurements. The donor heart sample parameters are consistent between the hearts, over time and with different operators indicating that Sydney Heart Bank donor hearts are a valid baseline control for comparison with pathological heart samples. We also discuss to what extent donor heart samples are representative of the normal heart.

References

Aug 1, 1996·Journal of Muscle Research and Cell Motility·S B MarstonG Roper
Nov 12, 2003·Journal of Muscle Research and Cell Motility·Steven Marston
Sep 24, 2004·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Martin Smith
Mar 14, 2007·Journal of Molecular and Cellular Cardiology·Eias JweiedPeter M Buttrick
Aug 12, 2008·Journal of Molecular and Cellular Cardiology·Steven B Marston, Pieter P de Tombe
Jan 14, 2009·Journal of Muscle Research and Cell Motility·Nazha HamdaniJolanda van der Velden
Mar 22, 2016·Biophysics Reviews·Sean LalCristobal Dos Remedios
Jul 27, 2018·Journal of Molecular and Cellular Cardiology·Wenxuan CaiYing Ge

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motility assay

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