Donor tissue preparation for Descemet membrane endothelial keratoplasty

Journal of Cataract and Refractive Surgery
Jessica T LieGerrit R J Melles

Abstract

To evaluate a technique for preparing a donor Descemet membrane carrying autologous endothelium for transplantation in Descemet membrane endothelial keratoplasty (DMEK). Netherlands Institute for Innovative Ocular Surgery, Rotterdam, The Netherlands. A 9.5 mm diameter DM carrying autologous endothelium was stripped from 10 corneoscleral rims that had been organ cultured for 1 week. The endothelial cell density (ECD) was evaluated with light microscopy before and immediately after DM was stripped and during 4 additional weeks of organ culture. The mean ECD was 2701 cells/mm(2) +/- 302 (SD) before and 2719 +/- 322 cells/mm(2) immediately after DM was stripped and declined from 2604 +/- 352 cells/mm(2) after 1 week to 2190 +/- 768 cells/mm(2) after an additional 4 weeks of organ culture (n = 10). Typical "endothelial streaks," ie, linear cellular disruptions observed immediately after DM was stripped, showed complete regeneration after the second culture period. Descemet grafts for transplantation in DMEK procedures can be surgically prepared from organ-cultured corneal rims and stored for an additional 3 weeks with acceptable endothelial cell loss. Because the donor tissue can be dissected directly from organ-cultured corneoscler...Continue Reading

References

Jun 1, 1982·Ophthalmology·W F Treffers
Mar 1, 1993·Journal of Cataract and Refractive Surgery·T M LangeB J McLaughlin
Sep 1, 1996·Survey of Ophthalmology·V P HoppenreijsW F Treffers
Nov 20, 1998·Cornea·G R MellesP S Binder
Mar 24, 1999·American Journal of Ophthalmology·G R MellesP S Binder
Mar 28, 2002·Cornea·Gerrit R J MellesCarla Nieuwendaal
Sep 25, 2003·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·G R J Melles, N Kamminga
Apr 16, 2004·Cornea·Gerrit R J MellesCarla P Nieuwendaal
Mar 1, 2005·American Journal of Ophthalmology·Teresa S IgnacioAshley Behrens
Apr 25, 2006·Journal of Cataract and Refractive Surgery·Francis W Price, Marianne O Price
Jun 30, 2006·Journal of Refractive Surgery·Gabriel van Rij, Marjolijn Bartels
Nov 15, 2006·Cornea·Gerrit R J Melles
Nov 15, 2006·Cornea·Gerrit R J MellesJacqueline van der Wees
Jul 25, 2007·Journal français d'ophtalmologie·E GardeaM Muraine
Dec 7, 2007·American Journal of Ophthalmology·Gerrit R J MellesJacqueline van der Wees
Feb 8, 2008·American Journal of Ophthalmology·Lisanne HamGerrit R J Melles
Mar 9, 2013·International Ophthalmology Clinics·Matthew T FengFrancis W Price

❮ Previous
Next ❯

Citations

Jun 23, 2009·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·P Maier, T Reinhard
Aug 21, 2012·Cornea·Efdal YoeruekBartz Schmidt
Nov 29, 2012·Cornea·Ruth QuilendrinoGerrit R J Melles
May 3, 2013·Cornea·Konstantinos DroutsasWalter Sekundo
May 7, 2009·Current Opinion in Ophthalmology·Isabel DapenaGerrit R J Melles
May 18, 2013·Current Opinion in Ophthalmology·Marianne O Price, Francis W Price
Jul 9, 2010·International Ophthalmology Clinics·Jennifer Y Li, Mark J Mannis
Jul 9, 2010·International Ophthalmology Clinics·Marianne O Price, Francis W Price
Mar 9, 2013·International Ophthalmology Clinics·William B LeeBruce Varnum
May 3, 2014·American Journal of Ophthalmology·Korine van DijkGerrit R J Melles
May 23, 2014·Advances in Therapy·George D KymionisAnastasios G P Konstas
Aug 8, 2014·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Anna-Karina B MaierNecip Torun
Apr 26, 2014·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·P P CiechanowskiG R J Melles
May 21, 2015·The British Journal of Ophthalmology·Marcus AngDonald Tan
Nov 30, 2013·Journal of Cataract and Refractive Surgery·Vasilios S LiarakosGerrit R J Melles
Aug 13, 2013·American Journal of Ophthalmology·Marc MuraineDavid Toubeau
Jul 23, 2013·Ophthalmology·Ursula Schlötzer-SchrehardtFriedrich E Kruse
Oct 31, 2012·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Korine van DijkGerrit R J Melles
Apr 24, 2012·Journal of Cataract and Refractive Surgery·Jack ParkerGerrit R J Melles
Apr 21, 2012·Survey of Ophthalmology·Arundhati AnshuFrancis W Price
Nov 30, 2010·Journal of Cataract and Refractive Surgery·Davide VenzanoCarlo Enrico Traverso
Aug 4, 2010·Ophthalmology·Massimo BusinDiego Ponzin
Oct 29, 2015·The British Journal of Ophthalmology·Jessica T LieGerrit R J Melles
Mar 3, 2009·Journal of Cataract and Refractive Surgery·Ioannis AthanasiadisBaddar M H Al Barwani
Sep 20, 2012·Acta Ophthalmologica·Martin DirisamerGerrit R J Melles
Jul 6, 2011·American Journal of Ophthalmology·Ludwig M HeindlClaus Cursiefen
Apr 15, 2014·Acta Ophthalmologica·Konstantinos DroutsasWalter Sekundo
Mar 30, 2010·American Journal of Ophthalmology·Bjoern O BachmannFriedrich E Kruse
Aug 28, 2015·Acta Ophthalmologica·Sherif A GamalElDinMalak I ElShazly

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.