Donor variability in HIV binding to peripheral blood mononuclear cells.

Virology Journal
Joshua J AnzingerGregory T Spear

Abstract

HIV infection of cells varies greatly between individuals, with multiple steps in the replication cycle potentially contributing to the variability. Although entry and post-entry variability of HIV infection levels in cells has been demonstrated, variability in HIV binding has not been examined. In this study, we examined variability of HIV binding to peripheral blood mononuclear cells (PBMC) from different donors. HIV binding to PBMC varied up to 3.9-fold between individuals and was independent of CD4. Replication of HIV in donor PBMC required CD4 and paralleled virus binding trends of donor PBMC. To assess the stability of virus binding phenotypes over time, HIV was bound to donors with low- and high-binding phenotypes. The binding phenotypes were maintained when tested weekly over a 4-week period for 3 of 4 donors, while one high-binding donor decreased to lower binding on the 4th week. The low- and high-binding phenotypes were also preserved across different HIV strains. Experiments performed to determine if there was an association between HIV binding levels and specific cell subset levels within PBMC showed no correlation, suggesting that HIV binds to multiple cell subsets. These results show that differences exist in HIV...Continue Reading

References

Jun 8, 1999·Annual Review of Immunology·E A BergerJ M Farber
Feb 28, 2002·AIDS Research and Human Retroviruses·Gene G OlingerGregory T Spear
Jul 2, 2002·Nature Medicine·Warner C Greene, B Matija Peterlin
Sep 28, 2002·Nature Immunology·Stuart G TurvilleAnthony L Cunningham
Mar 21, 2003·European Journal of Immunology·Deborah Greene Nguyen, James E K Hildreth
Jul 23, 2003·Biochimica Et Biophysica Acta·Stephen A GalloRobert Blumenthal
Jul 18, 2006·Virus Research·Joshua J AnzingerGregory T Spear
Nov 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lot de WittePhilippe Gallay

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Methods Mentioned

BETA
ELISA
flow cytometry

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