Donors of NO and pulsed radiation at lambda = 820 nm exert effects on cell attachment to extracellular matrices

Toxicology Letters
T KaruGalina S Kalendo

Abstract

The adhesion of HeLa cells to a glass matrix was evaluated after the irradiation of the cell suspension with a pulsed near-infrared light-emitting diode (lambda = 820 nm, frequency 10 Hz, dose 8-120 J/m(2)) and treatment with two donors of nitric oxide, sodium nitroprusside (SNP, 5 x 10(-4) M) and NaNO(2) (4 x 10(-4) M). It was found that in an irradiated cell suspension, the cell-glass adhesion increases in a dose-dependent manner (a bell-shaped curve with a maximum at 60 J/m(2)). The treatment of cells with SNP or NaNO(2) before the irradiation eliminates the radiation-induced attachment stimulation. Pretreatment of cells with SNP not only eliminates the radiation-induced attachment stimulation but inhibits the attachment of irradiated (but not non-irradiated) cells. It is suggested that a modulation of the activity of respiratory chain (probably the alteration of the activity of cytochrome c oxidase) is involved in radiation-induced increase of cell attachment.

References

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Jun 12, 1999·Journal of Photochemistry and Photobiology. B, Biology·T Karu

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Citations

Jul 25, 2008·Photochemistry and Photobiology·Tiina I Karu
Jan 8, 2008·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Brandon J ErdleAlice P Pentland
Mar 2, 2005·Lasers in Surgery and Medicine·Tiina I KaruNatalia I Afanasyeva
Jul 5, 2012·Annals of the New York Academy of Sciences·Tobias Jung, Tilman Grune
Aug 11, 2004·Journal of Theoretical Biology·Roland Thar, Michael Kühl

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