PMID: 2507339Sep 1, 1989Paper

Dopa and oxygen inhibit proliferation of retinal pigment epithelial cells, fibroblasts and endothelial cells in vitro

Experimental Eye Research
K AkeoC K Dorey

Abstract

Some quinones catalyze superoxide formation in a futile cycle involving electron transfer to molecular oxygen. If the quinolic precursors of melanin participated in the futile cycle, the high ambient oxygen surrounding postnatal RPE would make continued melanogenesis risk for the retina. To probe the possibility that arrest of melanogenesis in postnatal RPE is a protective mechanism, we assayed the growth rates of RPE cells, aortic endothelial cells and skin fibroblasts exposed to 0, 10, 50, 100 and 250 microM dopa. To assess the contribution of the futile cycle, we studied the effects of oxygen concentration and the antioxidants, superoxide dismutase and catalase. We found that all three cell types were significantly and dose-dependently inhibited by dopa and that the effects of dopa were oxygen dependent. The powerful inhibition of RPE cells by dopa was counteracted by inclusion of superoxide dismutase and catalase, but not by an inhibitor of dopa oxidase (phenylthiourea), indicating that the mechanism of growth suppression did not involve melanogenesis but, rather, dopa-dependent formation of superoxide in the media. Endothelial cells were more sensitive to the dopa-mediated oxidative damage than were RPE cells or fibroblast...Continue Reading

Citations

Jan 1, 1996·Documenta Ophthalmologica. Advances in Ophthalmology·A J AugustinS Hunt
Apr 2, 2005·The Journal of Comparative Neurology·Estela GiménezLluís Montoliu
Sep 28, 2005·Journal of Neurobiology·Ines Kralj-HansPeter Mobbs
Aug 1, 1992·Experimental Eye Research·B G Kennedy
Sep 23, 2008·Journal of Neuroscience Research·Jing YangMogens H Nissen
Aug 10, 2000·Journal of Veterinary Internal Medicine·A A Webb, G D Muir
Sep 26, 2019·Neural Regeneration Research·Alessio MartucciRaffaele Mancino

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