Dopachrome conversion factor functions as an isomerase

Biochemical and Biophysical Research Communications
J M Pawelek

Abstract

Dopachrome conversion factor is an enzymatic activity associated with the pigmentary system which catalyzes the conversion of dopachrome, an intermediate in melanin biosynthesis, to dihydroxyindole-2-carboxylic acid (DHICA). To date, the mechanism of action of DCF has been unknown because all previous assays have employed a dopachrome substrate contaminated with L-dopa. It has therefore not been possible to determine whether L-dopa acts as a hydrogen donor in the reaction or whether the formation of DHICA occurs through an isomerization of dopachrome. In this study it is shown that DCF catalyzes the conversion of dopachrome to DHICA equally well in the presence or absence of L-dopa. The DCF-mediated reaction thus appears to be an isomeric rearrangement of hydrogen ions from one portion of the dopachrome molecule to another. The results indicate that the name "dopachrome isomerase" appropriately describes the function of DCF.

References

Nov 1, 1989·The Journal of Investigative Dermatology·A K ChakrabortyM Ichihashi
Oct 1, 1988·Medicinal Research Reviews·G Prota
Aug 13, 1987·Biochimica Et Biophysica Acta·A PalumboG Prota
Feb 17, 1988·Biochimica Et Biophysica Acta·A PalumboT M Schultz
Jan 1, 1985·The Journal of Heredity·J I BarberR A King
Aug 1, 1984·The Journal of Investigative Dermatology·J I BarberR A King
Aug 1, 1980·The Journal of Investigative Dermatology·A M Körner, J Pawelek
Jul 1, 1982·The Journal of Investigative Dermatology·V J HearingJ M Pawelek

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Citations

Feb 19, 2000·Biochemical and Biophysical Research Communications·M Sugumaran, K Nellaiappan
Feb 1, 1993·Chemico-biological Interactions·D C ThompsonP Moldéus
Feb 22, 1995·Biochimica Et Biophysica Acta·A Sánchez-FerrerF García-Carmona
Jan 11, 1994·Biochimica Et Biophysica Acta·C SalinasJ A Lozano
Oct 25, 1995·Biochimica Et Biophysica Acta·E BeneditoJ C García-Borrón
Apr 5, 2005·Insect Biochemistry and Molecular Biology·A J Nappi, B M Christensen
Mar 1, 1997·Journal of Dermatological Science·K MaedaY Tomita
Mar 1, 1991·Pigment Cell Research·J M Pawelek
Nov 1, 1992·Pigment Cell Research·V J HearingI J Jackson
Dec 1, 1994·Pigment Cell Research·G OdhH Rorsman
Aug 1, 1995·Pigment Cell Research·M SugumaranC Amaratunga
Jan 1, 1997·Photochemistry and Photobiology·K Maeda, M Naganuma
Jan 10, 2002·Biochemical and Biophysical Research Communications·Jianmin FangJianyong Li
May 17, 2005·Pigment Cell Research·Kohsuke AdachiTakashi Hirata
Jul 13, 2013·Pigment Cell & Melanoma Research·Shosuke ItoKazumasa Wakamatsu
Sep 23, 2016·International Journal of Molecular Sciences·Manickam Sugumaran
Oct 25, 2016·International Journal of Molecular Sciences·Manickam Sugumaran, Hanine Barek
Nov 22, 2017·Pigment Cell & Melanoma Research·Hanine BarekKazumasa Wakamatsu
Mar 4, 1996·FEBS Letters·V del Marmol, F Beermann
May 11, 1992·FEBS Letters·A K ChakrabortyJ M Pawelek
Jan 5, 2011·Protein & Cell·Christopher J VavrickaJianyong Li
Jan 1, 1992·Pigment Cell Research·K TsukamotoV J Hearing
Nov 1, 1992·Pigment Cell Research·M Sugumaran
Sep 3, 2003·Pigment Cell Research·Anna Palumbo
Nov 11, 1999·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·K Valivittan
Apr 24, 2018·Frontiers in Molecular Biosciences·Anutthaman ParthasarathyAndré O Hudson
Jan 1, 1992·Pigment Cell Research·S J OrlowJ M Pawelek
Oct 8, 2020·International Journal of Molecular Sciences·Manickam SugumaranKazumasa Wakamatsu
Jan 27, 1999·Biochemical and Biophysical Research Communications·C Jiménez-CervantesJ C García-Borrón

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