Dopamine and Melamine Binding to Gold Nanoparticles Dominates Their Aptamer-Based Label-Free Colorimetric Sensing

Analytical Chemistry
Xixia LiuJuewen Liu

Abstract

Target-directed aptamer adsorption by gold nanoparticles (AuNPs) has been widely used to develop label-free colorimetric biosensors. However, the potential interactions between target molecules and AuNPs have not been considered, which may lead to misinterpretation of analytical results. In this work, the detection of dopamine, melamine, and K+ was studied as model systems to address this problem. First, dopamine and two control molecules all induced the aggregation of citrate-capped AuNPs with apparent Kd's of 5.8 μM dopamine, 51.6 μM norepinephrine, and 142 μM tyramine. Isothermal titration calorimetry measured the aptamer Kd to be 1.9 μM dopamine and 16.8 μM norepinephrine, whereas tyramine cannot bind. Surface enhanced Raman spectroscopy confirmed direct adsorption of dopamine, and the adsorbed dopamine inhibited the adsorption of DNA. Using a typical salt-induced colorimetric detection protocol, a similar color response was observed regardless of the sequence of DNA, indicating the observed color change reflected the adsorption of dopamine by the AuNPs instead of the binding of dopamine by the aptamer. For this label-free sensor to work, the interaction between the target molecule and AuNPs should be very weak, while dopam...Continue Reading

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Citations

Jul 18, 2020·The Analyst·Junling DuanJuewen Liu
Sep 30, 2020·Analytical Methods : Advancing Methods and Applications·Yangyang ChangJuewen Liu
Nov 3, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shingo Saito
Jan 25, 2021·Analytica Chimica Acta·Yongjie YangJuewen Liu
Feb 6, 2021·Sensors·Samy M Shaban, Dong-Hwan Kim
Mar 31, 2021·Chembiochem : a European Journal of Chemical Biology·Yaoyao HouXixia Liu
May 29, 2021·Critical Reviews in Food Science and Nutrition·Xiuguang XingWei Chen
Sep 11, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Jiaojiao ZhouJuewen Liu
Oct 1, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Fang Zhang, Juewen Liu
Oct 28, 2021·Angewandte Chemie·Obtin AlkhamisYi Xiao

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