Dopamine D2-like receptors are expressed in pancreatic beta cells and mediate inhibition of insulin secretion

The Journal of Biological Chemistry
B RubiP Maechler


Dopamine signaling is mediated by five cloned receptors, grouped into D1-like (D1 and D5) and D2-like (D2, D3 and D4) families. We identified by reverse transcription-PCR the presence of dopamine receptors from both families in INS-1E insulin-secreting cells as well as in rodent and human isolated islets. D2 receptor expression was confirmed by immunodetection revealing localization on insulin secretory granules of INS-1E and primary rodent and human beta cells. We then tested potential effects mediated by the identified receptors on beta cell function. Dopamine (10 microM) and the D2-like receptor agonist quinpirole (5 microM) inhibited glucose-stimulated insulin secretion tested in several models, i.e. INS-1E beta cells, fluorescence-activated cell-sorted primary rat beta cells, and pancreatic islets of rat, mouse, and human origin. Insulin exocytosis is controlled by metabolism coupled to cytosolic calcium changes. Measurements of glucose-induced mitochondrial hyperpolarization and ATP generation showed that dopamine and D2-like agonists did not inhibit glucose metabolism. On the other hand, dopamine decreased cell membrane depolarization as well as cytosolic calcium increases evoked by glucose stimulation in INS-1E beta cel...Continue Reading


Aug 1, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Paul E HarrisAntonella Maffei
Jan 24, 2009·Nature Clinical Practice. Endocrinology & Metabolism·Ritika R KapoorK Hussain
May 5, 2012·Nature Protocols·Rayner Rodriguez-DiazAlejandro Caicedo
Apr 18, 2009·Harvard Review of Psychiatry·Heather A KennaNatalie L Rasgon
Oct 18, 2008·Diabetes, Obesity & Metabolism·Matthew FreebyPaul E Harris
Sep 7, 2012·Diabetes, Obesity & Metabolism·Paul E Harris, Rudolph L Leibel
Aug 24, 2012·Molecular Endocrinology·Norman SimpsonPaul E Harris
Apr 25, 2008·The Journal of Endocrinology·Anne-Marie O'CarrollStephen J Lolait
Jun 16, 2011·Biomedical Research·Toshihiko IwanagaHiromi Takahashi-Iwanaga
Jun 18, 2010·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Masanori Ichise, Paul E Harris
Nov 13, 2013·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Teemu KalliokoskiMerja Haaparanta-Solin
Jan 1, 2014·Diabetology & Metabolic Syndrome·Michael EzrokhiAnthony H Cincotta
Dec 10, 2013·Nature Chemical Biology·Daisuke SakanoShoen Kume
Mar 8, 2014·Nuclear Medicine and Biology·Suresh PandeyJogeshwar Mukherjee
Sep 10, 2013·Endocrinology and Metabolism Clinics of North America·Chao Deng
Nov 27, 2015·Molecular Pharmaceutics·Stefanie M A WillekensMartin Gotthardt
Sep 4, 2015·Journal of Cellular Physiology·Eliana S Di CairanoCarla Perego
Oct 2, 2012·Seminars in Cell & Developmental Biology·Alejandro Caicedo
Jan 10, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Felicitas Lopez VicchiDamasia Becu-Villalobos
Mar 30, 2011·Medical Hypotheses·Bianca Lee Negrao, Margaretha Viljoen
Oct 1, 2008·PET Clinics·Mohamed HouseniAbass Alavi
Nov 9, 2007·Life Sciences·Maria KonstandiMatti A Lang
Dec 10, 2008·International Journal of Cancer. Journal International Du Cancer·Gwen MurphyElaine Lanza
Apr 12, 2016·Brain and Behavior·Susan E MartelleDon W Bowden
Apr 15, 2014·Diabetic Medicine : a Journal of the British Diabetic Association·B GuigasL M 't Hart
Feb 12, 2008·The American Journal of Medicine·Alan N Elias, Heather Hofflich


Sep 1, 1991·Pflügers Archiv : European journal of physiology·A DebuyserJ C Henquin
Mar 1, 1971·Clinical Pharmacology and Therapeutics·M H Van Woert, P S Mueller
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D M ShobackE M Brown
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M MemoI Hanbauer
Mar 1, 1993·The International Journal of Neuroscience·R Sandyk
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·E MezeyB J Hoffman
Jan 16, 1999·Molecular and Cellular Neurosciences·E V KuzhikandathilG S Oxford
Jul 29, 2000·Biochemical and Biophysical Research Communications·J VaughnP A Permana
Oct 4, 2000·Physiological Reviews·M E FreemanG Nagy
Nov 18, 2000·American Journal of Physiology. Cell Physiology·S NowickiA Aperia
Mar 7, 2001·The American Journal of Psychiatry·R Balon
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·R LinRobert Levenson
May 25, 2001·The Journal of Biological Chemistry·M ScarselliR Maggio
Aug 16, 2002·Molecular Pharmacology·Alicia V BindaRobert Levenson
Aug 24, 2002·Science·Noriko TakahashiHaruo Kasai
Sep 26, 2002·The Journal of Biological Chemistry·Natalie LavineHubert H M Van Tol
Dec 24, 2002·The Journal of Biological Chemistry·Wen GuoJonathan Javitch
Jan 4, 2003·Molecular Endocrinology·Mathias Z StrowskiHilary A Wilkinson
Mar 18, 2003·Nature Cell Biology·Hisamitsu IshiharaClaes B Wollheim
Apr 30, 2003·Journal of Neurochemistry·Yusuke Takeuchi, Kohji Fukunaga
Jul 25, 2003·Diabetologia·P Rorsman, E Renström
Nov 5, 2003·Biology of the Cell·Sophie CallierPhilippe Vernier
Nov 14, 2003·American Journal of Physiology. Endocrinology and Metabolism·Andrea SiegSusanne Ullrich
Jun 15, 2004·Nature Neuroscience·Günter U HöglingerEtienne C Hirsch

Related Concepts

Quinpirole Hydrochloride
Metabolic Process, Cellular
Establishment and Maintenance of Localization
Calcium [EPC]
Insulin Antagonists
Structure of Beta Cell of Islet
Mice, Inbred BALB C
Glucose Homeostasis
Mitochondrial Inheritance

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