Dopamine induces in vitro migration of synovial fibroblast from patients with rheumatoid arthritis.

Scientific Reports
Lina van NieSilvia Capellino

Abstract

Preventing synovial fibroblast (SF) migration into the adjacent cartilage is a desirable therapeutic target in rheumatoid arthritis (RA). As previous studies demonstrated that RASF and SF from osteoarthritis (OA) patients express dopamine receptors (DR), aim of the present study was to investigate the impact of dopamine on mobility of fibroblasts from patients with chronic arthritides. Synovial tissue and fibroblasts were obtained from RA and OA patients. Immunohistochemistry was performed for all DR-subtypes in the invasion zone. Migration- and motility-assays were performed under DR-stimulation. Cytokines were evaluated using ELISA. Expression of DRs was evaluated by flow cytometry, and DR activation was measured by xCELLigence real-time analysis. All DRs were expressed in RA invasion zone. Migration and motility of RASF and OASF were increased after DR stimulation in patients ≤ 75 years old. Synovial fibroblasts from older RA patients (> 75 years old) expressed lower levels of D1-, D2- and D4-DR than patients ≤ 75 years old. DR activation was not altered in older patients. Our results suggest a possible involvement of dopamine on migration of fibroblasts from arthritis patients. Therefore, the synovial dopaminergic pathway m...Continue Reading

References

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Citations

Apr 27, 2021·Frontiers in Immunology·Yifei Feng, Yan Lu
May 18, 2021·Frontiers in Molecular Biosciences·Yamina MohamediÁlvaro J Obaya

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Methods Mentioned

BETA
motility assay
FACS
FCS
scraping
ELISA

Software Mentioned

xCELLigence real time cell analysis ( RTCA )
RTCA
[UNK]
GraphPad Prism
ImageJ

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