Dopamine-mediated inhibition of renal Na+/K+-ATPase in HK-2 cells is reduced by ouabain

Clinical and Experimental Pharmacology & Physiology
Yurong Zhang, Zuyi Yuan

Abstract

1. Abnormal renal sodium handling is considered a major contributing factor in hypertension associated with chronic ouabain treatment. However, the molecular mechanisms involved in abnormal renal sodium handling have not been elucidated. Therefore, we investigated whether chronic ouabain treatment perturbs dopamine D(1) receptor function. 2. The expression and phosphorylation levels of the D(1) receptor in cells of the human proximal tubule cell line (HK-2) were determined using western blot analysis and reverse transcription polymerase chain reaction. The activity of the renal sodium/potassium pump (Na(+)/K(+)-ATPase) was measured using a colourimetric assay, and cyclic adenosine monophosphate accumulation was determined by performing a radioimmunoassay. 3. We showed that chronic ouabain treatment decreased the protein and mRNA expression levels of the D(1) receptor and increased the basal phosphorylation of the D(1) receptor in HK-2 cells. We also showed that in the presence of ouabain, HK-2 cells did not reveal the cyclic adenosine monophosphate accumulation and Na(+)/K(+)-ATPase inhibition induced by the D(1) receptor agonist fenoldopam. 4. We hypothesize that the ouabain-induced decrease in renal D(1) receptor function is ...Continue Reading

References

Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·C ChatziantoniouW J Arendshorst
Aug 1, 1993·Hypertension·C M YuanM B Pamnani
Feb 1, 1996·Journal of Hypertension·J M HamlynP Manunta
May 23, 1998·Annual Review of Pharmacology and Toxicology·J G Krupnick, J L Benovic
Dec 22, 1999·Journal of Cardiovascular Pharmacology·L V RossoniD V Vassallo
Feb 26, 2000·Acta Physiologica Scandinavica·R A FelderP A Jose
Sep 12, 2000·Current Hypertension Reports·P A JoseR A Felder
Dec 26, 2001·Current Opinion in Nephrology and Hypertension·Pedro A JoseRobin A Felder
Jan 5, 2002·Hypertension Research : Official Journal of the Japanese Society of Hypertension·G TianZ Lu
May 25, 2002·European Journal of Biochemistry·Wilhelm Schoner
Jul 12, 2002·American Journal of Physiology. Renal Physiology·Mohammad AsgharMustafa F Lokhandwala
Sep 5, 2002·Nature Reviews. Molecular Cell Biology·Kristen L PierceRobert J Lefkowitz
Oct 28, 2005·Current Pharmaceutical Design·M FerrandiP Ferrari
Mar 15, 2006·Cardiovascular & Hematological Agents in Medicinal Chemistry·Paolo ManuntaPatrizia Ferrari
May 15, 2007·American Journal of Physiology. Cell Physiology·Wilhelm Schoner, Georgios Scheiner-Bobis
Mar 19, 2008·Clinical and Experimental Pharmacology & Physiology·Alessandra S PadilhaDalton V Vassallo
Jul 25, 2009·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Yurong ZhangYanping Ren
Oct 22, 2009·Clinical and Experimental Pharmacology & Physiology·Peter Bie, Mads Damkjaer

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Citations

Feb 8, 2011·Nature Neuroscience·D Paola CalderonKamran Khodakhah
Nov 8, 2011·The Journal of Biological Chemistry·Diogo B PeruchettiCelso Caruso-Neves
Sep 26, 2013·Molecular Medicine Reports·Li-Nan ZhangZi-Bin Gao
May 24, 2013·American Journal of Physiology. Renal Physiology·Indira D PokkunuriMohammad Asghar
Jun 1, 2017·Oncology Reports·Xiang-Feng LiuHong-Liang Yao

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