Dopamine prevents nitration of tyrosine hydroxylase by peroxynitrite and nitrogen dioxide: is nitrotyrosine formation an early step in dopamine neuronal damage?

The Journal of Biological Chemistry
Samuel ParkD M Kuhn

Abstract

Peroxynitrite and nitrogen dioxide (NO2) are reactive nitrogen species that have been implicated as causal factors in neurodegenerative conditions. Peroxynitrite-induced nitration of tyrosine residues in tyrosine hydroxylase (TH) may even be one of the earliest biochemical events associated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced damage to dopamine neurons. Exposure of TH to peroxynitrite or NO2 results in nitration of tyrosine residues and modification of cysteines in the enzyme as well as inactivation of catalytic activity. Dopamine (DA), its precursor 3,4-dihydroxyphenylalanine, and metabolite 3,4-dihydroxyphenylacetic acid completely block the nitrating effects of peroxynitrite and NO2 on TH but do not relieve the enzyme from inhibition. o-Quinones formed in the reaction of catechols with either peroxynitrite or NO2 react with cysteine residues in TH and inhibit catalytic function. Using direct, real-time evaluation of tyrosine nitration with a green fluorescent protein-TH fusion protein stably expressed in intact cells (also stably expressing the human DA transporter), DA was also found to prevent NO2-induced nitration while leaving TH activity inhibited. These results show that peroxynitrite and NO2 reac...Continue Reading

References

Jan 1, 1992·Critical Reviews in Toxicology·T J Monks, S S Lau
Jul 1, 1995·Bioorganic & Medicinal Chemistry·M d'Ischia, C Costantini
Jan 1, 1994·Methods in Enzymology·J S BeckmanJ P Crow
Jan 1, 1993·Free Radical Research Communications·R E Huie, S Padmaja
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·T G HastingsM J Zigmond
Jan 1, 1996·Advances in Experimental Medicine and Biology·T G HastingsM J Zigmond
Nov 1, 1996·The American Journal of Physiology·J S Beckman, W H Koppenol
Mar 6, 1997·Biochemical and Biophysical Research Communications·A S PannalaS Singh
May 26, 1998·Journal of Neuropathology and Experimental Neurology·P F GoodC W Olanow
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·J AraH Ischiropoulos
Jul 31, 1998·Archives of Biochemistry and Biophysics·H Ischiropoulos
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·J V Ferrer, J A Javitch
Jun 1, 1997·Nitric Oxide : Biology and Chemistry·C DaveuC Ducrocq
Oct 9, 1998·The Journal of Biological Chemistry·S Pfeiffer, B Mayer
Nov 21, 1998·FEBS Letters·N Kerry, C Rice-Evans
Apr 29, 1999·Journal of Neuroscience Research·A H StokesK E Vrana
May 5, 1999·Current Opinion in Chemical Biology·J T Groves
Oct 19, 1999·Brain Research. Brain Research Reviews·F TorreillesJ Torreilles
Dec 3, 1999·Brain Research. Molecular Brain Research·D M Kuhn, R E Arthur
Mar 22, 2000·Chemical Research in Toxicology·J L BoltonT J Monks
Feb 22, 2001·Journal of Neurochemistry·R E WhiteheadJ B Justice
Jun 19, 2001·The Journal of Biological Chemistry·M G EspeyD A Wink
Oct 9, 2001·The Journal of Biological Chemistry·B Blanchard-FillionH Ischiropoulos
Nov 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S PfeifferB Mayer
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michael Graham EspeyDavid A Wink
Apr 30, 2002·Free Radical Biology & Medicine·Ohara AugustoSílvia L De Menezes
Jun 11, 2002·Methods : a Companion to Methods in Enzymology·John D Crispino, Stuart H Orkin
Aug 31, 2002·Journal of Neural Transmission·T Obata
Sep 5, 2002·Free Radical Biology & Medicine·Daniel RettoriEnrique Cadenas

❮ Previous
Next ❯

Citations

Sep 8, 2010·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Feng-Shiun ShieIng-Kang Ho
Jul 7, 2005·Antioxidants & Redox Signaling·Mahdieh SadidiDonald M Kuhn
Jun 30, 2009·Antioxidants & Redox Signaling·Vittorio CalabreseD Allan Butterfield
Jan 24, 2007·Physiological Reviews·Pál PacherLucas Liaudet
May 31, 2015·Neurobiology of Disease·Catherine A Cobb, Marsha P Cole
Apr 2, 2008·IUBMB Life·Enrico MonzaniLuigi Casella
Sep 23, 2003·Molecular Pharmacology·Donald M Kuhn, Timothy J Geddes
Apr 16, 2008·Journal of Cell Science·Mi-Yeon KimHee-Sae Park

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.