Dopamine receptor antagonists induce differentiation of PC-3 human prostate cancer cell-derived cancer stem cell-like cells

The Prostate
Su In LeeSong-Kyu Park

Abstract

The objective of this study was to determine whether PC-3 human prostate cancer cell-derived cancer stem cells (CSC)-like cells grown in a regular cell culture plate not coated with a matrix molecule might be useful for finding differentiation-inducing agents that could alter properties of prostate CSC. Monolayer cells prepared from sphere culture of PC-3 cells were characterized for the presence of pluripotency and tumorigenicity. They were then applied to screen a compound library to find compounds that could induce morphology changes of cells. Mechanisms of action of compounds selected from the chemical library that induced the loss of pluripotency of cells were also investigated. C5A cells prepared from PC-3 cell-derived sphere culture expressed pluripotency markers such as Oct4, Sox2, and Klf4. C5A cells were highly proliferative. They were invasive in vitro and tumorigenic in vivo. Some dopamine receptor antagonists such as thioridazine caused reduction of pluripotency markers and tumorigenicity. Thioridazine, unlike promazine, inhibited phosphorylation of AMPK in a dose dependent manner. BML-275, an AMPK inhibitor, also induced differentiation of C5A cells as seen with thioridazine whereas A769663, an AMPK activator, blo...Continue Reading

References

Feb 11, 1992·Trends in Pharmacological Sciences·D R Sibley, F J Monsma
Jul 4, 1990·Journal of the National Cancer Institute·P SkehanM R Boyd
Apr 2, 2005·Nature Reviews. Cancer·Michael DeanSusan Bates
May 12, 2005·Schizophrenia Research·Susanne Oksbjerg DaltonChristoffer Johansen
Dec 3, 2005·Cancer Research·Anne T CollinsNorman J Maitland
Sep 28, 2006·Annual Review of Medicine·Piero DalerbaMichael F Clarke
Nov 14, 2007·The Journal of Cell Biology·Tania HabibBrian M Iritani
Nov 21, 2007·Cancer Causes & Control : CCC·Ruth EtzioniEric Feuer
Feb 23, 2008·Nature Reviews. Cancer·Fiona M WattSalvador Aznar Benitah
Mar 23, 2010·The Journal of Urology·Kyung-Mi BaeDietmar W Siemann
Dec 28, 2010·Biochemical and Biophysical Research Communications·Nam Hyun KimYong Kee Kim
Jul 16, 2011·Hormones & Cancer·Norman J MaitlandAnne T Collins
Oct 15, 2011·International Journal of Clinical Practice·M KirbyE D Crawford
Mar 27, 2012·Cancer Letters·Yiwei LiFazlul H Sarkar
Jun 5, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Lilian E van VlerkenRobert E Hollingsworth
Aug 4, 2012·Critical Reviews in Eukaryotic Gene Expression·Chunyan YuEvan Keller
Jan 1, 2011·Genes·Francesca Soncin, Christopher M Ward
May 14, 2014·Drug Discovery Today·Panagiota A SotiropoulouDaniele Passarella
Apr 4, 2015·Seminars in Oncology·Jaffer A AjaniIra B Steinberg
Apr 30, 2017·Oncology Letters·Jiasheng MuHaineng Xu
Apr 30, 2017·Oncology Letters·Jiani ShenYu Yang
Jun 11, 2017·Oncology Letters·Hahn-Sun JungSong-Kyu Park
Jan 10, 2018·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Mar 21, 2018·Archives of Pharmacal Research·Md Saiful Islam Roney, Song-Kyu Park
Mar 23, 2018·International Journal of Molecular Sciences·Cameron A Wade, Natasha Kyprianou

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