Dopamine uptake dynamics are preserved under isoflurane anesthesia

Neuroscience Letters
Zachary D Brodnik, R A España

Abstract

Fast scan cyclic voltammetry is commonly used for measuring the kinetics of dopamine release and uptake. For experiments using an anesthetized preparation, urethane is preferentially used because it does not alter dopamine uptake kinetics compared to freely moving animals. Unfortunately, urethane is highly toxic, can induce premature death during experiments, and cannot be used for recovery surgeries. Isoflurane is an alternative anesthetic that is less toxic than urethane, produces a stable level of anesthesia over extended periods, and is often used for recovery surgeries. Despite these benefits, the effects of isoflurane on dopamine release and uptake have not been directly characterized. In the present studies, we assessed the utility of isoflurane for voltammetry experiments by testing dopamine signaling parameters under baseline conditions, after treatment with the dopamine uptake inhibitor cocaine, and after exposure to increasing concentrations of isoflurane. Our results indicate that surgical levels of isoflurane do not significantly alter terminal mechanisms of dopamine release and uptake over prolonged periods of time. Consequently, we propose that isoflurane is an acceptable anesthetic for voltammetry experiments, w...Continue Reading

References

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Mar 27, 2001·Annual Review of Pharmacology and Toxicology·T YamakuraR A Harris
Jan 29, 2002·Anesthesia and Analgesia·Koji Hara, R Adron Harris
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Oct 4, 2014·Frontiers in Neural Circuits·James E McCutcheonMitchell F Roitman

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Citations

Oct 30, 2016·NeuroImage·Ana M FiallosAlan Jasanoff
Dec 13, 2016·ACS Chemical Neuroscience·Zachary D BrodnikRodrigo A España
Oct 9, 2020·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·I P AlonsoR A España
May 7, 2021·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jessica K ShawRodrigo A España
Jul 9, 2021·Frontiers in Cellular Neuroscience·Angélica P EscobarPablo R Moya
Sep 27, 2018·ACS Chemical Neuroscience·Zachary D BrodnikRodrigo A España
Jun 12, 2019·ACS Chemical Neuroscience·Shaili AggarwalOle V Mortensen

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